Description

Acetylcholine-D9 Chloride is a stable isotope-labeled analog of the essential neurotransmitter acetylcholine, where nine hydrogen atoms are replaced by deuterium. This high-purity internal standard is critical for ensuring the accuracy and reliability of quantitative mass spectrometry analyses in complex biological matrices. It is primarily utilized by research scientists and quality control laboratories in the pharmaceutical, biotechnology, and neurochemical research sectors for method development, validation, and precise pharmacokinetic studies.

Application

• Quantitative Bioanalysis: Serves as a deuterated internal standard in LC-MS/MS and GC-MS methods for the accurate quantification of endogenous acetylcholine in plasma, cerebrospinal fluid, and tissue homogenates.
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables precise tracking of acetylcholine dynamics in preclinical and clinical research to understand drug effects on the cholinergic system.
• Neurological Disorder Research: Used in studies investigating Alzheimer's disease, Parkinson's disease, myasthenia gravis, and other conditions related to cholinergic signaling deficits.
• Drug Metabolism and Disposition: Facilitates the study of acetylcholinesterase inhibitor drugs and other compounds that interact with the cholinergic pathway.
• Method Development and Validation: Essential for developing robust, sensitive, and specific analytical methods in compliance with GLP and GMP guidelines.
• Quality Control in Pharmaceutical Manufacturing: Acts as a reference standard in the QC testing of drugs targeting the nervous system.

Basic Information

Product Name	Acetylcholine-D9 Chloride
CAS No.	344298-95-9
Molecular Formula	C7D9H7ClNO2 †
Molecular Weight	155.64 g/mol (for unlabeled base) †
Synonyms	(2-Acetoxyethyl)trimethyl-d9-ammonium Chloride; Deuterated Acetylcholine Chloride; ACh-d9; Acetylcholine (trimethyl-d9) Chloride; 2-(Acetyloxy)-N,N,N-trimethyl-d9-ethanaminium Chloride; (2-Acetyloxyethyl)trimethyl-d9-ammonium Chloride; N,N,N-Trimethyl-d9-2-acetoxyethylammonium Chloride
EINECS	Contact for details

† Calculated for unlabeled structure; exact mass for deuterated form available upon request.

Quality Control

Our Acetylcholine-D9 Chloride is manufactured under controlled conditions to ensure exceptional isotopic purity and chemical stability. Each batch undergoes rigorous analytical testing, including HPLC for chemical purity and MS for isotopic enrichment (typically ≥98 atom % D), to meet the exacting standards of analytical research. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or under an inert atmosphere to maintain stability and isotopic integrity. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms
Isotopic Purity (Atom % D)	≥98%
Chemical Purity (HPLC)	≥95%
Water Content (KF)	≤5.0%
Solubility	Soluble in water, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.