Description

Acetylcholine-1,1,2,2-D4 Chloride is a stable isotope-labeled analog of the essential neurotransmitter acetylcholine, where four hydrogen atoms are replaced by deuterium. This compound is critical for advanced research requiring precise quantification and metabolic pathway tracing in biological systems. It is primarily utilized by pharmaceutical R&D teams, academic researchers, and diagnostic developers in neuroscience, pharmacology, and metabolomics studies.

Application

• Used as an internal standard in Liquid Chromatography-Mass Spectrometry (LC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS) for the accurate quantification of acetylcholine in complex biological matrices.
• Essential for pharmacokinetic and metabolic studies to track the absorption, distribution, metabolism, and excretion of cholinergic drugs.
• Facilitates neurological research into cholinergic system function, related to memory, learning, and neuromuscular signaling.
• Employed in the development and validation of clinical diagnostic assays for conditions affecting cholinergic pathways.
• Supports drug discovery programs targeting acetylcholine receptors and enzymes like acetylcholinesterase.
• Applied in isotope dilution analysis to ensure high accuracy and precision in analytical measurements.

Basic Information

Product Name	Acetylcholine-1,1,2,2-D4 Chloride
CAS No.	344298-94-8
Molecular Formula	C7D4H12ClNO2
Molecular Weight	151.64 g/mol (for D4 isotopologue)
Synonyms	(2-Acetoxyethyl)trimethylammonium-1,1,2,2-d4 Chloride; Deuterated Acetylcholine Chloride; ACh-d4 Chloride; Acetylcholine-d4 Chloride; 2-(Acetyloxy)-N,N,N-trimethylethanaminium-1,1,2,2-d4 Chloride; (2-Acetyloxyethyl)trimethylammonium-1,1,2,2-d4 Chloride; ACh Cl-d4
EINECS	Contact for details

Quality Control

Every batch of Acetylcholine-1,1,2,2-D4 Chloride is manufactured and analyzed under strict quality protocols to ensure high chemical purity and isotopic enrichment suitable for sensitive analytical applications. Our quality commitment includes verification of identity, assay, and deuterium incorporation. A comprehensive Certificate of Analysis (COA) detailing lot-specific results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept in a dry environment and sealed immediately after use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder or solid
Identification (IR / NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (by NMR)	≥ 95%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.