Description

5-Bromo-4-Chloro-3-Indoxyl Thymidine-3'-Phosphate, Cyclohexylammonium Salt is a specialized chromogenic substrate used in enzymatic detection systems. Its core value lies in its ability to produce a highly visible, insoluble blue precipitate upon cleavage by specific phosphatases, making it indispensable for sensitive histochemical and molecular biology applications. This compound is primarily needed by researchers and manufacturers in the life sciences sector, particularly for developing diagnostic assays, conducting cellular localization studies, and in blotting techniques.

Application

• Histochemical Staining: Used as a substrate for alkaline phosphatase (AP) and other phosphatases in tissue sections to localize enzyme activity.
• Molecular Biology Assays: Employed in Western, Northern, and Southern blotting for the non-radioactive detection of nucleic acids and proteins.
• Diagnostic Kit Development: A key component in the formulation of clinical and research ELISA kits and other immunoassays requiring chromogenic detection.
• Microbiology: Used in chromogenic agar media for the identification and differentiation of bacterial colonies based on specific enzymatic activity.
• Cell Biology Research: Applied in studies of signal transduction and cellular processes involving phosphatase enzymes.
• In-situ Hybridization (ISH): Serves as a detection reagent for labeled probes in ISH protocols.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indoxyl Thymidine-3'-Phosphate, Cyclohexylammonium Salt
CAS No.	341973-00-0
Molecular Formula	C24H34BrClN4O11P
Molecular Weight	694.87 g/mol
Synonyms	BCIP Thymidine-3'-Phosphate Cyclohexylammonium Salt; 5-Bromo-4-chloro-3-indolyl thymidine 3'-phosphate; X-Phosphate (Thymidine Derivative); BCIP-TP; Thymidine 3'-(5-bromo-4-chloro-3-indolyl phosphate); Chromogenic Phosphatase Substrate (Thymidine); 3-Indoxyl phosphate, 5-bromo-4-chloro-, thymidine-3'-ester, cyclohexylammonium salt
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indoxyl Thymidine-3'-Phosphate, Cyclohexylammonium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light will degrade performance.

Specification

Item	Specification
Appearance	Off-white to light beige powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMF, DMSO; sparingly soluble in water
Functional Performance	Yields intense blue precipitate with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.