Description

P450 2E1 CAS NO 330196-93-5 is a highly purified, recombinant cytochrome P450 enzyme, specifically the 2E1 isoform, which plays a critical role in the metabolism of numerous xenobiotics and endogenous compounds. This enzyme is essential for researchers and developers in the pharmaceutical and chemical industries, providing a reliable tool for in vitro metabolic studies, toxicity screening, and drug-drug interaction assessments. It is primarily utilized by contract research organizations (CROs), academic research institutions, and pharmaceutical companies focused on ADME-Tox profiling and metabolic pathway elucidation.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used in in vitro assays to predict human metabolic clearance and identify primary metabolites.
• Toxicity Screening: Employed to assess the bioactivation potential of drug candidates and industrial chemicals into toxic intermediates.
• Enzyme Inhibition/Induction Studies: Critical for evaluating potential drug-drug interactions mediated by the CYP2E1 pathway.
• Biocatalysis and Synthetic Chemistry: Serves as a catalyst for specific oxidation reactions in the synthesis of fine chemicals and pharmaceutical intermediates.
• Biomarker Research: Used in studies investigating the role of CYP2E1 in diseases linked to oxidative stress and chemical exposure.
• Educational and Diagnostic Kit Development: Incorporated into laboratory kits for teaching advanced biochemistry and developing clinical diagnostic assays.

Basic Information

Product Name	P450 2E1
CAS No.	330196-93-5
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CYP2E1; Cytochrome P450 2E1; Cytochrome P450 Family 2 Subfamily E Member 1; P450 IIE1; P450-J; CYPIIE1; EC 1.14.14.1; Microsomal cytochrome P450 2E1; Recombinant human CYP2E1
EINECS	Contact for details

Quality Control

Our recombinant P450 2E1 is produced under stringent conditions to ensure high activity, purity, and batch-to-batch consistency. Each lot is thoroughly characterized for specific content and enzymatic activity using validated methods. Certificates of Analysis (COA) detailing purity, activity, and storage buffer composition are provided with every shipment to support your regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at ≤ -20°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use. Store in a cool, dry, and well-ventilated area.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Purity (SDS-PAGE)	≥ 90%
Specific Content (nmol P450/mg protein)	≥ 5
Activity (Substrate: p-Nitrophenol)	≥ 3.0 nmol/min/nmol P450
Protein Concentration	Provided on COA
Storage Buffer	50 mM Potassium Phosphate, pH 7.4, containing 20% (v/v) glycerol and 1 mM EDTA

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.