Description

6-[Fluorescein 6-Carboxamido]Hexanoic Acid is a high-purity, reactive derivative of fluorescein, designed for advanced bioconjugation and labeling applications. This compound is engineered to provide a stable, covalent linkage between biomolecules and the highly fluorescent fluorescein moiety, enabling sensitive detection and analysis. It is an essential reagent for researchers and manufacturers in the fields of diagnostic assay development, flow cytometry, fluorescence microscopy, and bioimaging probe synthesis.

Application

• Bioconjugation and Labeling: Primary reagent for covalently attaching fluorescent tags to antibodies, proteins, peptides, and oligonucleotides.
• Diagnostic Assay Development: Used in the production of fluorescent probes and tracers for ELISA, lateral flow assays, and other immunoassays.
• Cell Biology & Imaging: Synthesis of fluorescent probes for live-cell imaging, intracellular tracking, and fluorescence microscopy.
• Flow Cytometry: Creation of custom fluorescent antibody conjugates and cell surface markers for high-throughput cell analysis.
• Biomedical Research: Tool for studying protein-protein interactions, receptor-ligand binding, and other molecular events via fluorescence detection.
• Sensor Development: Key intermediate in the fabrication of fluorescent chemical sensors and biosensors for environmental or clinical monitoring.

Basic Information

Product Name	6-[Fluorescein 6-Carboxamido]Hexanoic Acid
CAS No.	323196-16-3
Molecular Formula	C31H22N2O8
Molecular Weight	550.52 g/mol
Synonyms	6-(Fluorescein-6-carboxamido)hexanoic acid; 6-FAM-C6-acid; Fluorescein-6-C6-acid; 5(6)-Carboxyfluorescein-C6-amide; N-(6-Carboxyhexyl)-5(6)-carboxyfluoresceinamide; 5(6)-FAM-C6-acid; 6-FAM hexanoic acid; Fluorescein derivative with C6 linker
EINECS	Contact for details

Quality Control

Our 6-[Fluorescein 6-Carboxamido]Hexanoic Acid is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and the absence of critical impurities like free fluorescein. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The product is light-sensitive and should be handled under subdued lighting where practical. For long-term storage, consider desiccation to maintain stability.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥95.0%
Free Fluorescein (HPLC)	≤1.0%
Water Content (KF)	≤5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.