Description

Myosin Light Chain Kinase (480-501) is a synthetic peptide fragment corresponding to a specific sequence of the myosin light chain kinase (MLCK) enzyme. This compound serves as a critical research tool for studying calcium/calmodulin-dependent signal transduction pathways and the regulation of smooth muscle contraction. It is primarily utilized by researchers and developers in the fields of biochemistry, cardiovascular research, and pharmaceutical discovery for use in enzyme assays, antibody production, and as a reference standard.

Application

• Biochemical Research: Used as a substrate or inhibitor in enzymatic assays to study Myosin Light Chain Kinase (MLCK) activity and regulation.
• Antibody Production: Serves as an immunogen for generating specific polyclonal or monoclonal antibodies targeting the MLCK enzyme.
• Signal Transduction Studies: A key reagent for investigating calcium/calmodulin-dependent pathways, particularly in smooth muscle and non-muscle cells.
• Cardiovascular & Pulmonary Research: Applied in studies focusing on vascular tone, hypertension, and bronchoconstriction mechanisms.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) assays to identify and characterize potential MLCK inhibitors for drug development.
• Cell Biology: Employed in experiments examining cytoskeletal dynamics, cell motility, and endothelial barrier function.

Basic Information

Product Name	Myosin Light Chain Kinase (480-501)
CAS No.	313642-00-1
Molecular Formula	C116H190N36O33S
Molecular Weight	2596.0 g/mol (approx.)
Synonyms	MLCK (480-501); Myosin Light Chain Kinase Peptide (480-501); MLCK Substrate Peptide; Calmodulin-dependent Myosin Light Chain Kinase Fragment; Smooth Muscle Myosin Light Chain Kinase Peptide; AV25; KKRAAKRAATSNVFS-amide (Sequence-based); MLCK Inhibitory Peptide
EINECS	Contact for details

Quality Control

Our Myosin Light Chain Kinase (480-501) is produced under stringent conditions to ensure batch-to-batch consistency and high biological activity. Each lot is subjected to comprehensive analytical testing, including HPLC for purity verification and mass spectrometry (MS) for identity confirmation. Certificates of Analysis (COA) detailing purity, sequence, and analytical data are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Amino Acid Sequence	KKRAAKRAATSNVFS-amide
Net Content	1 mg, 5 mg, 10 mg (or as requested)
Solubility	Soluble in water or dilute aqueous acetic acid

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.