Description

3-[(3-Cholamidopropyl)Dimethylammonio]-1-Propanesulfonate Monohydrate is a high-purity zwitterionic detergent widely recognized for its exceptional ability to solubilize membrane proteins while maintaining their native structure and function. This compound is critical for researchers and manufacturers requiring gentle yet effective protein extraction and stabilization. It is an essential reagent for biotechnology, pharmaceutical development, and advanced biochemical research applications.

Application

• Membrane Protein Solubilization: Primary application for extracting integral membrane proteins from lipid bilayers for structural and functional studies.
• Protein Biochemistry: Used in buffer systems for purifying and stabilizing proteins during chromatography (e.g., affinity, size-exclusion).
• Structural Biology: Essential for preparing samples for techniques like X-ray crystallography, cryo-electron microscopy (cryo-EM), and NMR spectroscopy.
• Diagnostic Assay Development: Employed as a stabilizing agent in immunoassays and other diagnostic kits to prevent protein aggregation.
• Cell Lysis: Effective component in lysis buffers for gentle disruption of cells and organelles.
• Vaccine and Therapeutic Protein Formulation: Aids in maintaining the solubility and stability of complex biologics during processing and storage.

Basic Information

Product Name	3-[(3-Cholamidopropyl)Dimethylammonio]-1-Propanesulfonate Monohydrate
CAS No.	313223-04-0
Molecular Formula	C32H58N2O7S • H2O
Molecular Weight	630.88 g/mol (Monohydrate)
Synonyms	CHAPS; 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonate hydrate; N,N-Dimethyl-3-sulfo-N-[3-(3α,7α,12α-trihydroxy-24-oxo-5β-cholan-24-yl)aminopropyl]propan-1-aminium inner salt monohydrate; Zwittergent 3-08; 3-[(3-Cholamidopropyl)dimethylammonio]-1-propanesulfonate; CHAPS Hydrate; 3-[(3-Cholamidopropyl)dimethylammonio]propanesulfonic acid inner salt monohydrate
EINECS	Contact for details

Quality Control

Our 3-[(3-Cholamidopropyl)Dimethylammonio]-1-Propanesulfonate Monohydrate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, to ensure it meets the high standards required for life science research and cGMP manufacturing. Certificates of Analysis (COA) with full traceability are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a desiccated environment to prevent absorption of moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0% (anhydrous basis)
Water Content (KF)	2.5 - 4.5 %
pH (1% Solution)	5.0 - 7.0
UV Absorbance (280 nm)	≤ 0.05 (1% solution, 1 cm path)
Solubility	Clear, colorless solution (10% in Water)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.