Description

[Asn18] Endothelin-1, Human is a synthetic, site-specific variant of the potent vasoconstrictor peptide Endothelin-1, where the native asparagine residue at position 18 is replaced with an aspartic acid. This modification is critical for structure-activity relationship (SAR) studies, enabling researchers to probe the role of specific amino acids in receptor binding and biological function. This high-purity peptide is an essential tool for cardiovascular research, pharmaceutical development, and biochemical assays targeting the endothelin system.

Application

• Biomedical Research: Fundamental studies on vasoconstriction, blood pressure regulation, and endothelial cell function.
• Drug Discovery & Development: Used as a reference standard and pharmacological probe in high-throughput screening (HTS) for endothelin receptor antagonists.
• Structure-Activity Relationship (SAR) Analysis: Critical for understanding the impact of specific amino acid substitutions on peptide potency and selectivity.
• In Vitro & Ex Vivo Assays: Functional characterization in cell-based assays (e.g., calcium flux, reporter gene) and isolated tissue bath experiments.
• Analytical Standard: Serves as a certified reference material (CRM) for method development and validation in HPLC and LC-MS analysis.
• Academic & Institutional Laboratories: Essential reagent for graduate-level research and published studies in pharmacology and physiology.

Basic Information

Product Name	[Asn18] Endothelin-1, Human
CAS No.	309245-97-4
Molecular Formula	C109H159N27O33S5
Molecular Weight	2492.9 g/mol
Synonyms	Endothelin-1 (18-Aspartic acid); ET-1 (Asn18Asp); Asp18-Endothelin-1; [Asp18]ET-1; Human Endothelin-1 (18-Asp) variant; H-Cys-Ser-Cys-Ser-Ser-Leu-Met-Asp-Lys-Glu-Cys-Val-Tyr-Phe-Cys-His-Leu-Asp-Ile-Ile-Trp-OH (Disulfide bridges: Cys1-Cys15 and Cys3-Cys11); EDN1 (18-Asp) peptide
EINECS	Contact for details

Quality Control

Our [Asn18] Endothelin-1, Human is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity assessment and MS for identity confirmation, to ensure it meets the exacting standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture absorption. Aliquot reconstituted solutions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Mass confirmed
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Water Content (KF)	≤ 5.0%
Acetate Content (HPLC)	≤ 10%
Solubility	Soluble in water, acetic acid, or DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.