Description

5-Bromo-4-Chloro-3-Indolyl Phosphate is a chromogenic substrate widely used in molecular biology and diagnostic applications. Its primary value lies in its enzymatic hydrolysis to produce an insoluble, intensely colored indigo dye, making it an essential reagent for sensitive detection. This compound is a critical component for researchers and manufacturers in the life sciences, biotechnology, and in vitro diagnostics (IVD) industries, particularly for applications in Western blotting, ELISA, and immunohistochemistry.

Application

• Western Blotting: Used as a substrate for alkaline phosphatase (AP)-conjugated antibodies to visualize protein bands.
• Enzyme-Linked Immunosorbent Assay (ELISA): Employed in colorimetric AP-based ELISA kits for the detection of antigens or antibodies.
• In Situ Hybridization (ISH): Serves as a chromogen for AP-labeled nucleic acid probes to localize specific DNA or RNA sequences.
• Immunohistochemistry (IHC): Applied for the colorimetric detection of tissue antigens using AP-conjugated primary or secondary antibodies.
• Lateral Flow Assays: Incorporated into rapid diagnostic test strips that utilize AP as a reporter enzyme.
• Research & Development: Utilized in various biochemical and cell-based assays requiring sensitive enzymatic detection.
• Diagnostic Reagent Manufacturing: A key raw material in the formulation of clinical diagnostic kits and reagents.

Basic Information

Product Name	5-Bromo-4-Chloro-3-Indolyl Phosphate
CAS No.	304656-10-8
Molecular Formula	C8H6BrClNO4P
Molecular Weight	326.47 g/mol
Synonyms	BCIP; 5-Bromo-4-chloro-3-indolyl phosphate; BCIP disodium salt; 5-Bromo-4-chloro-1H-indol-3-yl dihydrogen phosphate; X-Phosphate; Alkaline Phosphatase Substrate BCIP; 304656-10-8
EINECS	Contact for details

Quality Control

Our 5-Bromo-4-Chloro-3-Indolyl Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive life science applications. Certificates of Analysis (COA) detailing purity, impurity profile, and performance data are available for every lot upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMF, DMSO
Performance (Enzymatic)	Yields intense blue precipitate with Alkaline Phosphatase

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.