Description

Leucine Aminopeptidase, Porcine Kidney is a highly purified proteolytic enzyme that specifically catalyzes the hydrolysis of leucine and other hydrophobic amino acids from the N-terminus of peptides and proteins. This specificity makes it an indispensable tool for protein sequencing, peptide mapping, and biochemical research requiring precise N-terminal modification. It is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic industries who require reliable enzymatic activity for advanced proteomic analysis and quality control of biopharmaceuticals.

Application

• Protein Sequencing & Peptide Mapping: Used for the stepwise degradation of peptides from the N-terminus to determine amino acid sequences.
• Biopharmaceutical Analysis: Employed in the characterization and quality control of therapeutic proteins, including monoclonal antibodies, to assess N-terminal integrity.
• Proteomics Research: A key reagent for studying protein structure-function relationships, post-translational modifications, and protease specificity.
• Diagnostic Kit Manufacturing: Incorporated as a critical component in enzymatic assay kits for clinical diagnostics and biochemical testing.
• Food Science & Enzymology: Utilized in research to study protein hydrolysis and flavor development in food products.
• Substrate Specificity Studies: Serves as a model enzyme for investigating the kinetics and mechanism of aminopeptidase activity.

Basic Information

Product Name	Leucine Aminopeptidase, Porcine Kidney
CAS No.	300634-12-2
Molecular Formula	Contact for details
Molecular Weight	~54 kDa (Monomer)
Synonyms	LAP; Leucine Aminopeptidase (Microsomal); L-Leucine Aminopeptidase; Aminopeptidase M; Cytosol Aminopeptidase; EC 3.4.11.1; Leucyl Aminopeptidase; Kidney Aminopeptidase M
EINECS	Contact for details

Quality Control

Our Leucine Aminopeptidase, Porcine Kidney is produced and tested under stringent quality management systems. Each lot undergoes comprehensive analysis to ensure high specific activity, purity, and consistency suitable for research and industrial applications. Certificates of Analysis (COA) detailing assay, purity, and activity are provided with every shipment to guarantee compliance with your specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. The product is hygroscopic (moisture-sensitive). For long-term storage, consider desiccant or inert atmosphere to maintain optimal activity and stability. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms
Specific Activity	≥ 20 units/mg protein
Protein Content (Biuret)	≥ 90%
Purity (SDS-PAGE)	Single major band
Solubility	Clear, colorless solution in buffer
pH (in solution)	7.0 - 8.0
Loss on Drying	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.