Description

12β-Hydroxy-16β-Acetoxy-14,15β-Epoxy-3β-Hydroxy-5β-Bufa-20,22-Dienolide is a highly specialized bufadienolide derivative, a class of compounds known for their significant biological activity. This high-purity reference standard is critical for pharmaceutical research and development, particularly in the study of cardiac glycosides and novel therapeutic agents. It is primarily utilized by research institutions, pharmaceutical companies, and analytical laboratories engaged in drug discovery, metabolic studies, and quality control of complex natural products.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for the identification, assay, and purity testing of bufadienolide-based compounds in drug development and quality control laboratories.
• Drug Discovery & Medicinal Chemistry: Used as a key intermediate or building block in the synthesis and structure-activity relationship (SAR) studies of novel cardioactive or anticancer agents.
• Biochemical Research: Employed in in-vitro and in-vivo studies to investigate the mechanism of action, pharmacokinetics, and toxicology of cardiac glycosides and related natural products.
• Analytical Method Development: Essential for developing and validating precise analytical methods such as HPLC, LC-MS, and NMR for the quantification of complex steroidal compounds.
• Natural Product Isolation & Characterization: Acts as a critical comparative standard for the isolation, purification, and structural elucidation of similar compounds from natural sources like toad venom or medicinal plants.

Basic Information

Product Name	12β-Hydroxy-16β-Acetoxy-14,15β-Epoxy-3β-Hydroxy-5β-Bufa-20,22-Dienolide
CAS No.	39844-93-4
Molecular Formula	C₂₆H₃₄O₈
Molecular Weight	474.55 g/mol
Synonyms	16β-Acetoxy-12β,14β,15β-epoxy-3β-hydroxy-5β-bufa-20,22-dienolide; 12β,14β;15β-Epoxy-3β,16β-dihydroxy-5β-bufa-20,22-dienolide 16-acetate; Bufadienolide derivative; 14,15β-Epoxy-3β,12β,16β-trihydroxy-5β-bufa-20,22-dienolide 16-acetate; 12β-Hydroxy-16β-acetoxy-14,15β-epoxy-3β-hydroxy-5β-bufa-20,22-dienolide; 39844-93-4; Bufalin derivative; 16β-Acetoxy-12β-hydroxy-14,15β-epoxy-3β-hydroxy-5β-bufa-20,22-dienolide
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Quality Control

Our 12β-Hydroxy-16β-Acetoxy-14,15β-Epoxy-3β-Hydroxy-5β-Bufa-20,22-Dienolide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, NMR and IR for structural confirmation, and residual solvent analysis to ensure it meets the stringent requirements for research-grade materials. A detailed Certificate of Analysis (COA) documenting identity, purity, and all relevant specifications is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment and away from direct light exposure to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.