Description

DEAE Sephadex is a weak anion exchange chromatography medium derived from cross-linked dextran, functionalized with diethylaminoethyl groups. This product is essential for the separation and purification of a wide range of biomolecules, including proteins, nucleic acids, and enzymes, based on their charge characteristics. It is a critical tool for researchers and process developers in the biotechnology, pharmaceutical, and life sciences industries. The product is supplied as a dry, free-flowing powder and is identified by the CAS registry number 39455-31-7.

Application

• Protein Purification: Primary tool for the isolation and purification of enzymes, antibodies, and other charged proteins from complex biological mixtures.
• Nucleic Acid Separation: Used for the fractionation and purification of RNA, DNA, and oligonucleotides in molecular biology workflows.
• Enzyme Immobilization: Serves as a support matrix for the covalent attachment of enzymes, facilitating their use in biocatalysis and industrial processes.
• Desalting and Buffer Exchange: Effective for the removal of salts and small molecules from protein solutions via group separation.
• Process Development: Employed in downstream process development and scale-up for the production of biopharmaceuticals.
• Analytical Chromatography: Used in laboratory-scale analytical methods for the characterization of biomolecular charge variants.

Basic Information

Product Name	DEAE Sephadex
CAS No.	39455-31-7
Molecular Formula	(C6H10O5)n • (C6H14NCl)x
Molecular Weight	Polymeric substance
Synonyms	DEAE-Sephadex A-25; DEAE-Sephadex A-50; Diethylaminoethyl Sephadex; 2-Diethylaminoethyl ether of Sephadex; Dextran, 2-(diethylamino)ethyl ether, cross-linked; Sephadex, DEAE-; Anion exchanger based on cross-linked dextran; DEAE-Dextran gel
EINECS	254-467-5

Quality Control

Our DEAE Sephadex is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency and performance. Key parameters such as ion exchange capacity, particle size distribution, swelling behavior, and flow properties are strictly controlled. A Certificate of Analysis (COA) detailing the specifications and test results for each lot is provided to ensure traceability and compliance with your research or process requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive) and must be kept in a low-humidity environment to prevent caking and maintain its free-flowing properties and chromatographic performance.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Ion Exchange Capacity	3.5 ± 0.5 meq/g (dry weight)
Particle Size (wet)	40 - 120 μm (Fractionated)
Swelling (in water)	5 - 9 ml/g dry gel
pH Stability Range	2 - 10 (working)
Maximum Operating Pressure	0.3 MPa
Conductivity (in water)	< 1500 μS/cm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.