Description

Rubredoxin Clostridium Pasteurianum is a small, iron-sulfur redox protein isolated from the bacterium *Clostridium pasteurianum*. This compound is of significant interest in biochemical research and industrial biocatalysis due to its role in electron transfer processes, particularly in nitrogen fixation and other metabolic pathways. It serves as a critical reagent for enzymologists, molecular biologists, and researchers developing bio-inspired catalysts or studying metalloprotein function.

Application

• Biochemical Research: Used as a model protein for studying electron transfer mechanisms, protein folding, and the properties of iron-sulfur clusters.
• Enzymology Studies: Serves as an electron donor or acceptor in in vitro assays to investigate the activity of oxidoreductases and other redox-sensitive enzymes.
• Metalloprotein Analysis: A standard reference in spectroscopic studies (e.g., UV-Vis, EPR, Mössbauer) for characterizing metal coordination environments in proteins.
• Biocatalyst Development: Investigated for potential use in synthetic biology and industrial biocatalysis for driving redox reactions under mild conditions.
• Educational Tool: Employed in advanced biochemistry and biophysics laboratories to demonstrate principles of bioinorganic chemistry.
• Nitrogen Fixation Research: Used in studies related to the nitrogenase enzyme complex and biological nitrogen fixation pathways.

Basic Information

Product Name	Rubredoxin Clostridium Pasteurianum
CAS No.	39390-48-2
Molecular Formula	C~160H~260N~60O~160S4Fe (Approximate, protein)
Molecular Weight	~6,000 Da (Monomer, approximate)
Synonyms	Rubredoxin (Cp); Clostridium pasteurianum Rubredoxin; Ferredoxin (Rubredoxin type); Iron-sulfur protein (Rubredoxin); C. pasteurianum Rd; Electron transfer protein; [Fe(Cys)4] protein; Nitrogenase-associated protein
EINECS	Contact for details

Quality Control

Our Rubredoxin Clostridium Pasteurianum is produced and handled under controlled conditions to ensure batch-to-batch consistency and high biological activity. Each lot is characterized for purity, identity, and specific activity. Certificates of Analysis (COA) are provided, detailing parameters such as protein concentration (UV-Vis), purity (SDS-PAGE, HPLC), and iron content. We adhere to rigorous internal quality standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized (freeze-dried) material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -80°C to minimize repeated freeze-thaw cycles. The product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (UV-Vis)	Characteristic absorbance at ~490 nm
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford/UV)	≥ 90% of dry weight
Iron Content (AAS/ICP)	~1 atom Fe per molecule
Specific Activity	Contact for details
Solubility	Soluble in aqueous buffers (e.g., Tris, phosphate)
Endotoxin Level	< 1.0 EU/mg (if specified)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.