Description

Ribonuclease Inhibitor, Porcine is a highly purified protein that specifically and potently inhibits ribonucleases (RNases). This product is critical for protecting RNA integrity during sensitive molecular biology applications, preventing unwanted degradation that can compromise experimental results. It is an essential reagent for research and development laboratories in the life sciences, pharmaceutical, and biotechnology industries, particularly for applications involving RNA isolation, reverse transcription, in vitro transcription, and long-term RNA storage.

Application

• RNA Isolation and Purification: Added to lysis buffers to inhibit endogenous RNases during RNA extraction from tissues and cells.
• Reverse Transcription (RT) and RT-PCR: Protects RNA templates and newly synthesized cDNA from RNase contamination, ensuring accurate amplification.
• In Vitro Transcription: Safeguards synthesized RNA transcripts in mRNA production and other transcription-based systems.
• Long-term RNA Storage: Used as a stabilizing additive in RNA storage solutions to maintain sample integrity over time.
• Nuclease-Free Reagent Preparation: Essential for creating nuclease-free environments and reagents for sensitive molecular biology workflows.
• Biopharmaceutical Manufacturing: Supports processes in mRNA vaccine and therapeutic development where RNA stability is paramount.

Basic Information

Product Name	Ribonuclease Inhibitor, Porcine
CAS No.	39369-21-6
Molecular Formula	Contact for details
Molecular Weight	~50 kDa (Protein)
Synonyms	Porcine RNase Inhibitor; RNase Inhibitor from Porcine Liver; Ribonuclease Inhibitor (Porcine); RNase Inactivator; RNasin (alternative trade name); Ribonuclease Inhibitor Protein; RNase Inhibitor Protein; RNase Inhibitor, Type I
EINECS	Contact for details

Quality Control

Our Ribonuclease Inhibitor, Porcine is manufactured under stringent conditions to ensure high activity and purity. Each lot undergoes rigorous quality testing, including functional activity assays, purity assessment, and tests for contaminating nuclease activities. A Certificate of Analysis (COA) detailing specific results for purity, activity (units/mg), and absence of DNase/Protease is provided with every shipment to guarantee batch-to-batch consistency and performance for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Clear, colorless solution
Identification (Activity)	Inhibits RNase A activity
Assay (Activity)	≥ 20,000 units/mg protein
Purity (SDS-PAGE)	≥ 95%
DNase Activity	Not detected
Protease Activity	Not detected
pH	7.5 - 8.5
Buffer	50 mM HEPES-KOH, 50 mM KCl, 5 mM DTT, 50% Glycerol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.