Description

Aphidicolin CAS NO 38966-21-1 is a potent, cell-permeable tetracyclic diterpenoid antibiotic isolated from the fungus *Cephalosporium aphidicola*. This compound is a highly specific and reversible inhibitor of eukaryotic DNA polymerases α and δ, making it an invaluable tool for synchronizing cells in the G1/S phase of the cell cycle. It is essential for researchers in molecular biology, oncology, and virology, as well as for pharmaceutical development targeting DNA replication.

Application

• Cell Cycle Research & Synchronization: Used to reversibly arrest eukaryotic cells at the G1/S phase boundary, enabling the study of cell cycle progression and checkpoint controls.
• Oncology & Cancer Research: Employed to inhibit DNA replication in cancer cell lines, facilitating studies on tumor cell proliferation, apoptosis, and the efficacy of chemotherapeutic agents.
• Virology Studies: Utilized to investigate the replication mechanisms of DNA viruses (e.g., herpesviruses, poxviruses) by selectively inhibiting host nuclear DNA synthesis.
• Biochemical Assay Standard: Serves as a critical reference standard and inhibitor in enzymatic assays for DNA polymerase activity and fidelity.
• Pharmaceutical Development: Acts as a lead compound or biochemical tool in drug discovery programs aimed at developing novel antiviral or anticancer therapeutics.

Basic Information

Product Name	Aphidicolin
CAS No.	38966-21-1
Molecular Formula	C20H34O4
Molecular Weight	338.48 g/mol
Synonyms	Aphidicolin; NSC 234714; Antibiotic 10054; (1R,3aS,5aR,5bR,7aR,9S,11aR,11bS,13aR,13bS)-3a,5a,5b,8,8,11a-Hexamethyl-1-prop-1-en-2-yl-1,2,3,4,5,6,7,7a,9,10,11,11b,12,13,13a,13b-hexadecahydrocyclopenta[a]chrysen-9-ol; 3α,16β,17-Trihydroxyaphidicolane
EINECS	Contact for details

Quality Control

Our Aphidicolin is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high purity for research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for fine chemicals and biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. The product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Biological Activity	Inhibits DNA polymerase α/δ (IC50 consistent with reference)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.