Description

Mtt Formazan CAS NO 23305-68-2 is a vital tetrazolium salt derivative widely used as a key reagent in biochemical assays. Its primary value lies in its ability to produce a measurable colorimetric signal upon reduction by metabolically active cells, making it indispensable for quantifying cell viability and proliferation. This product is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors who require reliable and sensitive assay components.

Application

• Cell Viability and Proliferation Assays: The MTT assay, utilizing MTT formazan, is a gold-standard method for measuring mitochondrial activity in live cells, critical for drug screening and cytotoxicity studies.
• Anticancer Drug Discovery: Used in high-throughput screening to evaluate the efficacy and IC50 values of novel chemotherapeutic compounds on various cancer cell lines.
• Toxicology Testing: Applied in environmental and industrial toxicology to assess the cytotoxic effects of chemicals, nanomaterials, and pollutants on mammalian cells.
• Microbiology Research: Employed to study microbial cell growth and metabolic activity, including antifungal and antibacterial susceptibility testing.
• Biomaterial and Implant Testing: Used to evaluate the biocompatibility of medical devices, scaffolds, and polymers by measuring cell adhesion and growth on material surfaces.
• Academic and Basic Research: A fundamental tool in cell biology, immunology, and biochemistry laboratories for routine cell culture analysis and experimental validation.

Basic Information

Product Name	Mtt Formazan
CAS No.	23305-68-2
Molecular Formula	C₁₈H₁₆N₈
Molecular Weight	344.37 g/mol
Synonyms	MTT Formazan; Thiazolyl Blue Formazan; 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide formazan; MTT Reduction Product; MTT Tetrazolium Formazan; MTT-Formazan; Tetrazolium Blue Formazan; Thiazolyl Blue Tetrazolium Formazan
EINECS	Contact for details

Quality Control

Our Mtt Formazan is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive bioassays. Each lot undergoes rigorous analytical testing, including HPLC for purity and spectral analysis for identity confirmation, to meet the exacting standards required for life science research. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance characteristics, and performance in a standard MTT assay is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive (store away from light) and should be handled to minimize prolonged exposure to ambient conditions.

Specification

Item	Specification
Appearance	Dark blue to purple crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Absorbance (in DMSO)	λmax ~ 570 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffers
Loss on Drying	≤ 1.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.