Description

2,3-Bis(4-Fluorophenyl)-5-Phenyltetrazolium Chloride is a specialized tetrazolium salt derivative, a critical reagent in biochemical and pharmaceutical research. Its primary value lies in its role as a precursor or intermediate in the synthesis of formazan dyes, which are widely used as indicators in cell viability and cytotoxicity assays. This high-purity compound is essential for researchers and manufacturers in the life sciences, analytical chemistry, and pharmaceutical development sectors who require reliable, well-characterized building blocks for diagnostic and research applications.

Application

• Biochemical Research Reagent: Key intermediate in the synthesis of formazan dyes for MTT and related cell proliferation/viability assays.
• Pharmaceutical Intermediate: Used in the research and development of novel therapeutic agents and diagnostic tools.
• Analytical Chemistry: Serves as a chromogenic substrate or indicator in enzymatic and colorimetric detection methods.
• Life Science Diagnostics: Component in the formulation of kits for measuring metabolic activity in live cells.
• Material Science Research: Potential use in the development of specialized dyes and sensors.

Basic Information

Product Name	2,3-Bis(4-Fluorophenyl)-5-Phenyltetrazolium Chloride
CAS No.	135788-09-9
Molecular Formula	C25H15ClF2N4
Molecular Weight	444.86 g/mol
Synonyms	2,3-Bis(p-fluorophenyl)-5-phenyltetrazolium chloride; 2,3-Di(4-fluorophenyl)-5-phenyl-2H-tetrazolium chloride; 5-Phenyl-2,3-bis(4-fluorophenyl)tetrazolium chloride; Tetrazolium Red Chloride (related analog); TTC derivative; Formazan dye precursor; MTT analog intermediate
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Fluorophenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods (NMR, IR), to ensure it meets the high standards required for research and development. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive); therefore, the container must be kept tightly sealed in a dry environment to prevent degradation. For long-term storage, consider desiccants or inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.