Description

2,3-Diphenyl-5-(2-Thienyl)Tetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its core value lies in its ability to produce a measurable formazan dye upon reduction, making it indispensable for quantifying cellular metabolic activity and enzymatic function. This compound is primarily needed by research institutions, pharmaceutical developers, and diagnostic manufacturers for applications in cell viability testing, cytotoxicity screening, and dehydrogenase enzyme assays.

Application

• Cell Viability and Proliferation Assays (e.g., MTT assay alternatives): Used to measure the metabolic activity of living cells in culture.
• Microbiological Testing: Employed as an indicator for bacterial growth and metabolic activity in various media.
• Enzyme Activity Assays: Serves as a substrate for dehydrogenase enzymes, enabling the quantification of enzymatic reactions.
• Cytotoxicity and Drug Screening: Utilized in high-throughput screening to assess the toxic effects of chemical compounds on cells.
• Biochemical Research: Acts as an electron acceptor in studies of mitochondrial function and oxidative stress.
• Diagnostic Reagent Formulation: A key component in kits for clinical and research diagnostics.

Basic Information

Product Name	2,3-Diphenyl-5-(2-Thienyl)Tetrazolium Chloride
CAS No.	38800-20-3
Molecular Formula	C23H17ClN4S
Molecular Weight	416.92 g/mol
Synonyms	Thiazolyl Blue Tetrazolium Chloride (common alias); MTT Tetrazolium (related analog); 2,3,5-Triphenyl-2H-tetrazolium chloride (structural analog name); TPTZ; Tetrazolium Red; INT (2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium chloride) - related compound; Nitro Blue Tetrazolium (NBT) - related compound; Thiazolyl Blue
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-(2-Thienyl)Tetrazolium Chloride is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR spectroscopy, to ensure it meets high-purity standards suitable for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed in a desiccated environment and away from direct light exposure to maintain stability and potency.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMSO, methanol; sparingly soluble in water
Heavy Metals	< 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.