Description

2,3-Diphenyl-5-(p-Diphenyl)Tetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator in biochemical assays. Its core value lies in its ability to form a colored formazan product upon reduction, making it an essential reagent for measuring cell viability, proliferation, and cytotoxicity. This compound is primarily needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences industries for high-throughput screening and metabolic activity studies.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in MTT and related assays to quantify metabolically active cells.
• Cytotoxicity Testing: Employed in pharmaceutical development to screen the toxic effects of new drug candidates on cell cultures.
• Microbiological Analysis: Applied in microbiology for assessing bacterial dehydrogenase activity and microbial growth.
• Enzyme Activity Studies: Serves as an electron acceptor in assays measuring the activity of various oxidoreductase enzymes.
• Antioxidant Capacity Assays: Utilized in biochemical tests to evaluate the free radical scavenging potential of compounds.
• High-Throughput Screening (HTS): A critical component in automated HTS platforms for drug discovery and biochemical research.

Basic Information

Product Name	2,3-Diphenyl-5-(p-Diphenyl)Tetrazolium Chloride
CAS No.	37632-83-0
Molecular Formula	C31H23ClN4
Molecular Weight	487.00 g/mol
Synonyms	MTT Tetrazolium Salt; MTT; Thiazolyl Blue Tetrazolium Bromide (common related name); 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium Bromide (common related IUPAC); Tetrazolium Chloride, 2,3-Diphenyl-5-(p-diphenyl)-; p-Diphenyltetrazolium Chloride; MTT Reagent; Tetrazolium Blue (related compound)
EINECS	Contact for details

Quality Control

Our 2,3-Diphenyl-5-(p-Diphenyl)Tetrazolium Chloride is produced and tested under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a dry environment to prevent absorption of moisture, which can affect stability and performance. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Solubility	Soluble in DMSO, ethanol; sparingly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.