Description

Ec 5.3.4.1 CAS NO 37318-49-3 is a high-purity enzyme preparation, specifically identified as Protein Disulfide Isomerase (PDI). This compound is critical for catalyzing the formation, reduction, and isomerization of disulfide bonds within proteins, a fundamental process for proper protein folding and function. It is an essential reagent for researchers and manufacturers in the biotechnology, pharmaceutical, and life science sectors who require reliable and active enzymes for protein engineering, therapeutic protein production, and biochemical research.

Application

• Biopharmaceutical Manufacturing: Used in the production and refolding of recombinant therapeutic proteins, including monoclonal antibodies and complex biologics, to ensure correct three-dimensional structure and bioactivity.
• Biochemical Research & Development: Serves as a key tool for studying protein folding mechanisms, disulfide bond dynamics, and endoplasmic reticulum function in cell-free and cellular systems.
• In Vitro Diagnostics (IVD): Employed in the development of diagnostic assays and kits where controlled protein folding or activity is required for accurate detection.
• Enzyme Engineering: Acts as a target or a helper enzyme in studies focused on improving the stability and function of industrial enzymes through disulfide bond introduction.
• Cell Culture & Fermentation: Can be added to cell culture media to enhance the yield of properly folded secreted proteins in mammalian and microbial expression systems.

Basic Information

Product Name	Ec 5.3.4.1 CAS NO 37318-49-3
CAS No.	37318-49-3
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Protein Disulfide Isomerase; PDI; EC 5.3.4.1; Disulfide Isomerase; Prolyl 4-hydroxylase subunit beta; Cellular Thyroid Hormone-Binding Protein; p55; Glycoprotein III; Procollagen-Proline, 2-Oxoglutarate 4-Dioxygenase Subunit Beta; 37318-49-3
EINECS	Contact for details

Quality Control

Our Ec 5.3.4.1 is produced under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and consistency. Certificates of Analysis (COA) detailing parameters such as activity units/mg, purity by SDS-PAGE, and endotoxin levels are available upon request. We adhere to relevant guidelines for research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or as specified on the label. This product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or solution
Identification (IR / Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported on COA
Endotoxin	< 1.0 EU/μg (for cell culture grade)
Solubility	Soluble in appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.