Description

3Alpha,7Alpha,12Alpha-Trihydroxy-5Beta-Cholan-24-Oylglycine Amidohydrolase is a specialized enzyme critical for biochemical research and diagnostic applications. This compound matters as a key reagent for studying bile acid metabolism and related enzymatic pathways. It is primarily needed by pharmaceutical R&D laboratories, diagnostic kit manufacturers, and academic institutions conducting metabolic research.

Application

• Biochemical Research: Used as a substrate or reference standard in enzymatic assays to study bile salt hydrolase activity and gut microbiome function.
• Diagnostic Development: Serves as a critical component in the development of clinical diagnostic tests for liver function and metabolic disorders.
• Pharmaceutical R&D: Employed in drug discovery programs targeting enzymes involved in cholesterol and bile acid homeostasis.
• Enzyme Characterization: Utilized to investigate enzyme kinetics, inhibition, and specificity of amidohydrolase enzymes.
• Metabolic Pathway Analysis: Aids in the elucidation of complex biochemical pathways related to lipid digestion and absorption.
• Quality Control Reference: Acts as a high-purity reference material for calibrating analytical instruments and validating methods in QC laboratories.

Basic Information

Product Name	3Alpha,7Alpha,12Alpha-Trihydroxy-5Beta-Cholan-24-Oylglycine Amidohydrolase
CAS No.	37289-07-9
Molecular Formula	C26H43NO6
Molecular Weight	465.63 g/mol
Synonyms	Glycocholic Acid Amidohydrolase; Cholylglycine Hydrolase; Bile Salt Hydrolase (BSH) Substrate; N-Choloylglycine Hydrolase; 3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine Amidohydrolase; Glycocholate Hydrolase; Conjugated Bile Acid Hydrolase; EC 3.5.1.24 (related activity)
EINECS	Contact for details

Quality Control

Our 3Alpha,7Alpha,12Alpha-Trihydroxy-5Beta-Cholan-24-Oylglycine Amidohydrolase is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic use. Certificates of Analysis (COA) detailing purity, assay results, and impurity profiles are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. Due to its hygroscopic (moisture-sensitive) nature, the container must be kept sealed in a desiccated environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 98.0% (on dried basis)
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.