Description

Pi-Plc, 1-Phosphatidyl-D-Myo-Inositol Inositol Phosphohydrolase, Cyclic-Phosphate Forming is a highly specific phospholipase C enzyme that catalyzes the hydrolysis of phosphatidylinositol, a key reaction in cellular signal transduction pathways. This enzyme is critical for research into intracellular signaling mechanisms, particularly those involving inositol phosphate second messengers. It is an essential biochemical tool for pharmaceutical R&D, academic research in cell biology, and diagnostic assay development.

Application

• Biochemical Research: A fundamental tool for studying phosphoinositide-specific phospholipase C (PI-PLC) activity, inositol phosphate metabolism, and related signal transduction cascades.
• Pharmaceutical Development: Used in drug discovery and screening assays targeting enzymes in the phosphatidylinositol pathway, which is implicated in various diseases.
• Diagnostic Reagent Manufacturing: Incorporated into enzymatic assay kits for the detection and quantification of specific phospholipids or related metabolites in clinical and research settings.
• Enzymology Studies: Employed to investigate enzyme kinetics, substrate specificity, and inhibitor effects on PI-PLC enzymes.
• Cell Signaling Analysis: Utilized to manipulate cellular levels of diacylglycerol (DAG) and inositol phosphates to dissect downstream effects in model systems.
• Production of Biochemical Standards: Used to generate specific inositol phosphate or diacylglycerol products for use as analytical standards or labeled probes.

Basic Information

Product Name	Pi-Plc, 1-Phosphatidyl-D-Myo-Inositol Inositol Phosphohydrolase, Cyclic-Phosphate Forming
CAS No.	37288-19-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Phosphatidylinositol Phospholipase C; PI-PLC; 1-Phosphatidyl-1D-myo-inositol inositolphosphohydrolase (cyclic-phosphate-forming); Inositol Phosphodiesterase; Phosphatidylinositol Inositolphosphohydrolase; Phosphoinositide Phospholipase C; PtdIns-PLC; EC 3.1.4.11
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Each batch is characterized for specific activity, purity, and stability to guarantee performance in sensitive applications. Certificates of Analysis (COA) detailing assay results, protein concentration, and unit definition are available upon request. Production can adhere to relevant guidelines for research and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive). For optimal preservation, aliquot into single-use vials under inert atmosphere to minimize freeze-thaw cycles and moisture uptake. Keep containers sealed in a dry, cool environment.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Catalyzes hydrolysis of Phosphatidylinositol
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Concentration	Defined per batch (Bradford/Lowry)
Stability	Retains ≥ 90% activity after 12 months at -20°C
Buffer Composition	Defined per formulation (e.g., Tris, Glycerol)
Endotoxin Level	Available upon request for specific grades

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.