Description

Endo-β-n-Acetylglucosaminidase F2 is a highly specific endoglycosidase enzyme that catalyzes the cleavage of the chitobiose core of high-mannose and hybrid type N-linked glycans. This enzymatic precision is critical for the structural analysis and controlled modification of glycoproteins, a cornerstone of modern biopharmaceutical development. It is an essential tool for researchers and manufacturers in biotechnology, pharmaceuticals, and diagnostics who require reliable and high-purity enzymes for glycoprotein characterization, therapeutic protein engineering, and glycan-based assay development.

Application

• Glycoprotein Structural Analysis: Used for deglycosylation to simplify mass spectrometry analysis and determine glycosylation sites.
• Biopharmaceutical Development: Critical for the characterization and quality control of monoclonal antibodies and other therapeutic glycoproteins.
• Glycoengineering: Enables the remodeling of antibody Fc glycans to modulate effector functions like Antibody-Dependent Cellular Cytotoxicity (ADCC).
• Diagnostic Reagent Manufacturing: Employed in the production of components for glycan-based diagnostic kits and biosensors.
• Enzymatic Synthesis: Serves as a biocatalyst for the chemo-enzymatic synthesis of defined glycan structures and glycoconjugates.
• Cell Biology Research: Aids in studying the role of specific N-glycans in cellular processes, including protein folding, trafficking, and cell signaling.

Basic Information

Product Name	Endo-β-n-Acetylglucosaminidase F2
CAS No.	37278-88-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Endo F2; Endoglycosidase F2; Endo-β-N-acetylglucosaminidase F2; N-Glycanase F2; PNGase F2 (common misnomer); Endo-F2; Endo H-F2 (specificity variant); EC 3.2.1.96
EINECS	Contact for details

Quality Control

Our Endo-β-n-Acetylglucosaminidase F2 is produced and tested under stringent quality management systems. Each lot undergoes rigorous analytical testing to ensure high specific activity, purity, and absence of contaminating exoglycosidase or protease activities. A comprehensive Certificate of Analysis (COA) detailing purity, activity units, and stability data is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation. For short-term use, aliquoting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Activity)	Cleaves high-mannose and hybrid N-glycans
Specific Activity	> XX U/mg (Contact for details)
Purity (SDS-PAGE)	> 95%
Endotoxin Level	< 1.0 EU/μg (for research-grade)
Protease Activity	Not detected
Exoglycosidase Activity	Not detected
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.