Description

Ec 1.5.1.11 CAS NO 37256-27-2 is a high-purity enzyme preparation, specifically identified as 5,10-Methylenetetrahydrofolate reductase (MTHFR). This compound is a critical biocatalyst in folate metabolism and one-carbon transfer cycles, essential for nucleotide synthesis and amino acid metabolism. It is primarily utilized by research institutions, diagnostic manufacturers, and biotechnology companies engaged in biochemical research, enzymatic assay development, and metabolic pathway studies.

Application

• Biochemical Research: Used as a key reagent in studies of folate metabolism, homocysteine regulation, and one-carbon unit transfer mechanisms.
• Diagnostic Kit Manufacturing: Serves as a critical component in enzymatic assay kits for the clinical measurement of homocysteine, folate, and related metabolites.
• Enzyme Kinetics Studies: Employed to investigate enzyme activity, inhibition, and kinetics for academic and pharmaceutical research purposes.
• Pharmaceutical Development: Used in screening and development of potential therapeutic agents targeting MTHFR-related disorders.
• Nutritional Science Research: Applied in studies examining the impact of vitamins (B2, B6, B9, B12) on enzyme function and metabolic health.
• Reference Standard: Acts as a purified standard for quality control and calibration in related analytical methods.

Basic Information

Product Name	Ec 1.5.1.11
CAS No.	37256-27-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	5,10-Methylenetetrahydrofolate Reductase; MTHFR; Methylenetetrahydrofolate Reductase (NADPH); EC 1.5.1.20; 5-Methyltetrahydrofolate:NADP+ Oxidoreductase; 5,10-CH2-H4folate Reductase; NADPH-Dependent Methylenetetrahydrofolate Reductase
EINECS	Contact for details

Quality Control

Our Ec 1.5.1.11 is produced and tested under strict quality management systems. Each batch undergoes rigorous analysis to ensure high specific activity, purity, and stability, meeting the stringent requirements for research and diagnostic applications. Certificates of Analysis (COA) detailing activity units/mg protein, purity by SDS-PAGE, and functional stability are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is typically supplied in a buffered glycerol solution. Refer to the specific Certificate of Analysis for detailed storage and stability information.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Identification (Activity)	Conforms (Specific activity provided on COA)
Specific Activity	> X U/mg protein (Batch-specific on COA)
Purity (SDS-PAGE)	> 90% (Single major band)
Protein Concentration	X.X - X.X mg/mL (Determined by Bradford/UV)
Buffer Composition	Tris/HCl, Glycerol, DTT (or similar stabilizers)
pH	7.0 - 8.0
Stability	≥ 12 months at -20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.