Description

Sarcosine Dehydrogenase is a critical enzyme that catalyzes the oxidation of sarcosine to glycine, formaldehyde, and hydrogen peroxide. This reaction is fundamental to one-carbon metabolism and is essential for research and diagnostic applications requiring precise measurement of sarcosine or related metabolites. It is primarily utilized by biotechnology firms, diagnostic kit manufacturers, and academic research institutions focused on metabolic studies, biomarker discovery, and enzymatic assay development.

Application

• Clinical Diagnostics: Key component in enzymatic assay kits for the quantitative determination of sarcosine in serum and urine, a biomarker of interest in prostate cancer research.
• Biochemical Research: Used as a model enzyme in studies of mitochondrial metabolism, one-carbon cycle dynamics, and flavoprotein dehydrogenase mechanisms.
• Enzyme Engineering: Serves as a target for protein engineering and stability studies to develop more robust enzymatic reagents for industrial biocatalysis.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify potential inhibitors or modulators of sarcosine metabolism for therapeutic intervention.
• Food & Beverage Analysis: Used in enzymatic methods for the detection and quantification of sarcosine and related compounds in food quality control and nutritional analysis.

Basic Information

Product Name	Sarcosine Dehydrogenase
CAS No.	37228-65-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sarcosine Dehydrogenase (Recombinant); Sarcosine:acceptor oxidoreductase (demethylating); EC 1.5.99.1; SarDH; SARDH; Glycine dehydrogenase; Mitochondrial sarcosine dehydrogenase
EINECS	Contact for details

Quality Control

Our Sarcosine Dehydrogenase is produced under stringent quality management systems. Each lot undergoes rigorous testing for specific activity, purity, and stability to ensure consistent performance in sensitive applications. A comprehensive Certificate of Analysis (COA) detailing assay results, protein concentration, and storage buffer information is provided with every shipment to guarantee traceability and reliability for your research and production needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive) and light-sensitive; handle under appropriate conditions to maintain optimal enzymatic activity.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Specific Activity	≥ XX U/mg protein
Protein Concentration	XX mg/mL (by Bradford)
Purity	≥ 95% (by SDS-PAGE)
Identification (Activity)	Conforms (Sarcosine-specific oxidation)
pH	7.0 - 8.0
Buffer	XX mM Tris-HCl, XX mM NaCl, XX% Glycerol
Endotoxin Level	< 1.0 EU/µg (for diagnostic grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.