Description

5-Iodo-3-Indolyl-β-D-Galactopyranoside is a chromogenic substrate used for the detection of β-galactosidase activity. It matters because it provides a highly sensitive and visually distinct blue-colored precipitate upon enzymatic cleavage, enabling precise localization and quantification in various assays. This compound is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, and molecular biology sectors who require reliable histochemical staining and reporter gene detection.

Application

• Histochemical Staining: Primary substrate for visualizing β-galactosidase expression in tissues, cells, and bacterial colonies (e.g., blue-white screening).
• Reporter Gene Assays: Critical for lacZ gene expression studies in molecular biology and genetic engineering.
• Enzyme Activity Detection: Used in blotting techniques (Western, Southern) and in situ hybridization to detect enzymatic activity.
• Microbiological Testing: Employed in diagnostic kits for identifying specific bacterial strains based on β-galactosidase production.
• Biochemical Research: Tool for studying gene regulation, promoter activity, and cell lineage tracing in developmental biology.
• Pharmaceutical Screening: Utilized in high-throughput screening assays to identify modulators of gene expression.

Basic Information

Product Name	5-Iodo-3-Indolyl-β-D-Galactopyranoside
CAS No.	36473-36-6
Molecular Formula	C14H16INO6
Molecular Weight	421.19 g/mol
Synonyms	5-Bromo-4-chloro-3-indolyl-β-D-galactopyranoside (X-gal) analog; Iodoindolyl galactoside; 5-Iodo-3-indolyl-β-D-galactoside; Galacto-iodoindoxyl; IBG; Bluo-Gal; 36473-36-6; β-Galactosidase chromogenic substrate (iodo derivative)
EINECS	Contact for details

Quality Control

Our 5-Iodo-3-Indolyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMF, DMSO; slightly soluble in water
Melting Point	Contact for details
Heavy Metals	< 10 ppm
Biological Activity	Suitable for β-galactosidase detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.