Description

Dihydrofluorescein Diacetate CAS NO 35340-49-9 is a cell-permeable, non-fluorescent ester derivative widely used as a critical reagent in oxidative stress research. Its core value lies in its ability to be hydrolyzed by intracellular esterases and subsequently oxidized by reactive oxygen species (ROS) to produce the highly fluorescent compound, fluorescein. This mechanism makes it an indispensable tool for researchers and quality control professionals in the pharmaceutical, biotechnology, and life sciences industries who require sensitive and reliable detection of cellular oxidative activity.

Application

• Intracellular ROS Detection: Primary application for measuring reactive oxygen species (ROS) such as hydrogen peroxide, peroxynitrite, and hydroxyl radicals in live cells.
• Oxidative Stress Assays: Used in high-throughput screening (HTS) platforms to evaluate the pro-oxidant or antioxidant effects of pharmaceutical compounds, nutraceuticals, and environmental toxins.
• Pharmaceutical R&D: Employed in drug discovery and development to study mechanisms of action, cytotoxicity, and side effects related to oxidative pathways.
• Biomedical Research: A key reagent in studies of apoptosis, inflammation, neurodegenerative diseases, and cardiovascular disorders where oxidative stress is a contributing factor.
• Diagnostic Kit Manufacturing: Serves as a core component in the formulation of commercial assay kits for research and clinical diagnostics.
• Quality Control in Cell Biology: Used to validate cell viability and monitor the oxidative state of cell cultures in bioproduction and standard laboratory practice.

Basic Information

Product Name	Dihydrofluorescein Diacetate
CAS No.	35340-49-9
Molecular Formula	C24H18O7
Molecular Weight	418.40 g/mol
Synonyms	2',7'-Dichlorodihydrofluorescein diacetate; DCFH-DA; H2DCFDA; 2',7'-Dichlorofluorescin diacetate; Dihydrodichlorofluorescein diacetate; 5(6)-Carboxy-2',7'-dichlorofluorescein diacetate; Reactive Oxygen Species Detection Probe; Oxidative Stress Indicator
EINECS	Contact for details

Quality Control

Our Dihydrofluorescein Diacetate is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric moisture. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm
Fluorescence Suitability	Pass performance test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.