Description

Hypoxanthine-[2,8-3H] is a tritium-labeled derivative of the purine base hypoxanthine, specifically radiolabeled at the 2 and 8 positions. This compound is an essential tool for researchers requiring high-specific-activity tracers in metabolic and biochemical studies. It is primarily used by scientists in pharmaceutical R&D, academic biochemistry, and molecular biology laboratories investigating nucleotide metabolism, enzyme kinetics, and nucleic acid synthesis pathways.

Application

• Biochemical Tracer Studies: Used as a radiolabeled precursor to track the synthesis and turnover of purine nucleotides and nucleic acids (DNA/RNA).
• Enzyme Assay Development: Serves as a substrate for enzymes like xanthine oxidase and hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in kinetic and inhibition studies.
• Metabolic Pathway Analysis: Enables the detailed mapping of salvage and *de novo* purine biosynthesis pathways in cell cultures and tissue samples.
• Drug Discovery & Pharmacology: Utilized in high-throughput screening assays to evaluate the efficacy of potential therapeutic agents targeting purine metabolism, such as anticancer and antiparasitic drugs.
• Autoradiography & Imaging: Provides the radioactive signal for techniques used to visualize the localization and distribution of purine metabolites in biological systems.

Basic Information

Product Name	Hypoxanthine-[2,8-3H]
CAS No.	33917-71-4
Molecular Formula	C5H4N4O
Molecular Weight	136.11 g/mol (unlabeled)
Synonyms	1,7-Dihydro-6H-purin-6-one, [2,8-3H]; 6-Hydroxypurine, tritiated; Purin-6(1H)-one, 1,9-dihydro-, [2,8-3H]; [2,8-3H]Hypoxanthine; Tritiated hypoxanthine; 3H-Hypoxanthine
EINECS	Contact for details

Quality Control

Every batch of Hypoxanthine-[2,8-3H] is manufactured and analyzed under strict quality systems to ensure traceability and reliability for sensitive research applications. Product identity, radiochemical purity, and specific activity are verified using advanced analytical techniques including HPLC with radiometric detection. A comprehensive Certificate of Analysis (COA) detailing batch-specific results is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store the product at or below -20°C in the original solvent as supplied. Store in accordance with all local and national regulations for radioactive materials.

Specification

Item	Specification
Appearance	Clear solution
Identification (HPLC)	Conforms
Radiochemical Purity	≥ 97%
Specific Activity	20-40 Ci/mmol
Chemical Purity (HPLC)	≥ 98%
Solvent	Ethanol:Water (7:3) or as specified

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.