Description

5-Bromoindoxyl Diacetate is a specialized chromogenic substrate used as a key reagent in enzymatic and histochemical assays. Its primary value lies in its ability to produce an insoluble, colored precipitate upon enzymatic hydrolysis, making it an essential tool for visualizing and quantifying specific enzyme activity. This compound is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic kit manufacturing sectors for applications ranging from cell biology to high-throughput screening.

Application

• Enzyme Substrate for β-Galactosidase (LacZ) Assays: Widely used in molecular biology and genetics as a histochemical stain to detect β-galactosidase activity, commonly in reporter gene systems (e.g., lacZ gene expression).
• Microbiological Testing: Employed in diagnostic media and kits for the identification of specific bacterial enzymes, aiding in microbial differentiation and pathogen detection.
• Neuroscience and Cell Biology Research: Utilized in tissue staining to map and study the expression patterns of specific enzymes within neural and other tissue samples.
• High-Throughput Screening (HTS): Serves as a critical reagent in drug discovery platforms to identify modulators of glycosidase enzyme activity.
• Diagnostic Kit Manufacturing: An essential raw material in the production of clinical and veterinary diagnostic tests that rely on chromogenic detection methods.
• Plant Biology Studies: Applied in research to study gene expression and enzyme localization in transgenic plants.

Basic Information

Item	Detail
Product Name	5-Bromoindoxyl Diacetate
CAS No.	33588-54-4
Molecular Formula	C12H10BrNO4
Molecular Weight	312.12 g/mol
Synonyms	5-Bromo-3-indoxyl diacetate; 5-Bromoindoxyl acetate; 3-(Acetyloxy)-5-bromo-1H-indol-2-yl acetate; 5-Bromo-3-hydroxyindole diacetate; 5-Br-Indoxyl diacetate; BID; 5-Bromo-3-indolyl diacetate; 5-Bromo-3-indoxyl diacetate ester
EINECS	Contact for details

Quality Control

Our 5-Bromoindoxyl Diacetate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your critical workflows.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive (store away from light). Keep the container tightly sealed in a dry environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Loss on Drying	≤ 0.5%
Heavy Metals	≤ 20 ppm
Residual Solvents	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.