Description

4A-Hydroxy-5-Methyltetrahydrofolic Acid is a key folate derivative and a critical intermediate in one-carbon metabolism. This compound matters for its role in the biosynthesis of nucleotides and amino acids, making it essential for research into cellular processes and metabolic pathways. It is needed by pharmaceutical R&D teams, academic researchers, and manufacturers of high-purity biochemicals for applications in drug discovery, nutritional science, and advanced biochemical synthesis.

Application

• Pharmaceutical Research & Development: As a reference standard and intermediate in the synthesis of novel folate-based therapeutics and metabolic modulators.
• Biochemical Research: Used in enzymatic studies and metabolic pathway analysis to investigate one-carbon transfer reactions.
• Nutritional Science: Serves as a high-purity compound for studying folate metabolism, bioavailability, and the development of advanced nutritional supplements.
• Diagnostic Reagent Manufacturing: Employed in the production of precise assay kits and calibrators for clinical diagnostics related to folate status and metabolic disorders.
• Academic & Institutional Laboratories: A vital reagent for fundamental research in biochemistry, molecular biology, and cell biology.
• Precursor for Advanced Synthesis: Used as a building block for the chemical synthesis of more complex, labeled, or conjugated folate molecules.

Basic Information

Product Name	4A-Hydroxy-5-Methyltetrahydrofolic Acid
CAS No.	33157-07-2
Molecular Formula	C₂₀H₂₅N₇O₇
Molecular Weight	475.46 g/mol
Synonyms	5-Methyl-5,6,7,8-tetrahydrofolic acid; 5-Methyltetrahydrofolate; 5-Methyl-THF; (6S)-5-Methyl-5,6,7,8-tetrahydrofolic acid; L-5-Methyltetrahydrofolate; 5-Methyl H4 folate; Metafolin (brand name reference); 5-CH3-THF
EINECS	Contact for details

Quality Control

Our 4A-Hydroxy-5-Methyltetrahydrofolic Acid is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets exacting standards for research and development use. A comprehensive Certificate of Analysis (COA) detailing all test results is provided with every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as specified on the label or COA. For long-term stability, storage at -20°C under an inert atmosphere is recommended. Keep the container dry and in a well-ventilated place.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (HPLC/UV)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Purity (HPLC, Area %)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.