Description

5,10-Methylene-THF is a crucial folate coenzyme derivative and a key intermediate in one-carbon metabolism. This compound matters for its role in the biosynthesis of nucleotides, amino acids, and other essential biomolecules, making it vital for research and development in life sciences. It is primarily needed by pharmaceutical companies, academic research institutions, and biotechnology firms focused on metabolic studies, drug discovery, and the development of novel therapeutic agents.

Application

• Biochemical Research: As a critical cofactor in enzymatic reactions involving one-carbon transfer, essential for studying folate metabolism and related pathways.
• Pharmaceutical Intermediates: Serves as a key building block in the synthesis of complex drug molecules, particularly those targeting metabolic diseases and cancers.
• Enzyme Activity Studies: Used in vitro to investigate the kinetics and mechanisms of enzymes like thymidylate synthase and methylenetetrahydrofolate reductase (MTHFR).
• Nutritional Science: Employed in research to understand the bioavailability and metabolic fate of synthetic and natural folates.
• Diagnostic Reagent Development: A potential component in the formulation of assay kits for measuring folate levels or related enzyme activities in clinical samples.
• Cell Culture & Microbiology: May be used as a supplement in defined media for the growth of fastidious microorganisms or specialized cell lines requiring specific folate forms.

Basic Information

Product Name	5,10-Methylene-THF
CAS No.	31690-11-6
Molecular Formula	C20H23N7O6
Molecular Weight	457.44 g/mol
Synonyms	5,10-Methylenetetrahydrofolic Acid; 5,10-CH2-THF; 5,10-Methylene-5,6,7,8-tetrahydrofolic Acid; 5,10-Methylene-H4 folate; NSC 139490; (6S)-5,10-Methylenetetrahydrofolate; 5,10-Methylene-THFA; 5,10-Methylene-5,6,7,8-tetrahydrofolate
EINECS	Contact for details

Quality Control

Our 5,10-Methylene-THF is produced under strict quality management protocols to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for assay and purity, and is supported by a detailed Certificate of Analysis (COA). We adhere to relevant quality standards suitable for research and development applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) due to its sensitivity to moisture and potential oxidation. Allow the sealed container to reach room temperature before opening to minimize condensation and exposure to air.

Specification

Item	Specification
Appearance	Off-white to light yellow powder or solid
Identification (HPLC/UV)	Conforms
Assay (HPLC)	≥ 95.0%
Purity (HPLC)	≥ 98.0% (Area %)
Water Content (KF)	≤ 2.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.