Description

Nocodazole CAS NO 31430-18-9 is a synthetic, cell-permeable benzimidazole derivative that acts as a potent microtubule polymerization inhibitor. This compound is a critical research tool for cell biology, enabling the study of cell cycle dynamics, mitotic spindle function, and intracellular transport mechanisms. It is essential for researchers and professionals in pharmaceutical R&D, biotechnology, and academic laboratories focused on cancer research, cytogenetics, and drug discovery.

Application

• Cell Cycle Synchronization: Used to reversibly arrest cells in the G2/M phase of the cell cycle for synchronized population studies.
• Microtubule Dynamics Research: A standard laboratory reagent for investigating microtubule assembly, disassembly, and their role in cellular structure.
• Cancer and Anti-mitotic Drug Research: Serves as a reference compound in screening and developing novel chemotherapeutic agents that target the mitotic spindle.
• Cytogenetics: Employed in chromosome spreading techniques and karyotype analysis by disrupting the mitotic spindle.
• Cell Biology Studies: Facilitates research on intracellular organelle transport, cell morphology, and membrane trafficking processes dependent on microtubules.
• Biochemical Assays: Used in in vitro tubulin polymerization assays to study inhibitor kinetics and mechanisms.

Basic Information

Product Name	Nocodazole
CAS No.	31430-18-9
Molecular Formula	C₁₄H₁₁N₃O₃
Molecular Weight	301.26 g/mol
Synonyms	Methyl [5-(2-thienylcarbonyl)-1H-benzimidazol-2-yl]carbamate; Oncodazole; R 17934; NSC 238159; TJF 033; 5-(2-Thienylcarbonyl)-1H-benzimidazol-2-yl carbamic acid methyl ester; Antimitotic agent R 17934
EINECS	250-630-7

Quality Control

Our Nocodazole is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and residual solvent analysis. We provide full traceability and Certificates of Analysis (COA) are available for every lot to ensure compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and potency.

Specification

Item	Specification
Appearance	White to pale yellow crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.