Description

4-Vinylpyridoxal-5-Phosphate is a chemically modified, biologically active derivative of pyridoxal phosphate (PLP), the active coenzyme form of vitamin B6. This compound is valued for its vinyl group, which provides a reactive handle for conjugation, cross-linking, and immobilization onto various matrices. It is primarily utilized by researchers and manufacturers in the fields of biochemistry, enzymology, and pharmaceutical development for studying enzyme mechanisms, developing diagnostic assays, and creating affinity chromatography media.

Application

• Enzyme Mechanism Studies: Acts as a coenzyme analog or inhibitor to probe the active sites and catalytic pathways of PLP-dependent enzymes like transaminases and decarboxylases.
• Affinity Chromatography Ligand: Immobilized onto solid supports (e.g., agarose beads) for the purification of specific proteins and enzymes that bind pyridoxal phosphate.
• Diagnostic Reagent Synthesis: Serves as a key intermediate in the development of fluorescent or colorimetric probes and assay kits for clinical and research diagnostics.
• Bioconjugation and Cross-linking: The vinyl group enables covalent attachment to other biomolecules (proteins, peptides) or surfaces for creating stable complexes in biophysical studies.
• Pharmaceutical Intermediate: Used in the research and development of novel therapeutic agents targeting metabolic pathways involving vitamin B6.
• Biochemical Research Standard: Employed as a reference standard in analytical laboratories for quality control and method validation.

Basic Information

Product Name	4-Vinylpyridoxal-5-Phosphate
CAS No.	31383-64-9
Molecular Formula	C12H14NO7P
Molecular Weight	315.22 g/mol
Synonyms	4-Vinylpyridoxal phosphate; 4-Vinyl-PLP; 3-Hydroxy-5-(hydroxymethyl)-2-methyl-4-vinylpyridine-4-carbaldehyde 5-(Dihydrogen phosphate); 4-Ethenyl-3-hydroxy-5-(hydroxymethyl)-2-methylisonicotinaldehyde 5-phosphate; PLP vinyl derivative; Vitamin B6 coenzyme analog
EINECS	Contact for details

Quality Control

Our 4-Vinylpyridoxal-5-Phosphate is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation, to ensure it meets high-purity research standards. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic (moisture-sensitive) nature, the container should be kept in a desiccated environment. For long-term storage, consider storing under an inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.