Description

Metrizamide is a non-ionic, iodinated contrast medium widely recognized for its utility in density gradient centrifugation. This compound is valued for its high solubility in aqueous solutions and its ability to form stable, iso-osmotic gradients critical for the separation of biological particles. It is an essential reagent for researchers and laboratories in the life sciences, particularly for the isolation of cells, subcellular organelles, viruses, and nucleic acids. Its application ensures high purity and viability of separated biological materials.

Application

• Density gradient medium for the isolation of lymphocytes, monocytes, and other mammalian cells.
• Separation and purification of subcellular organelles, including mitochondria, lysosomes, and peroxisomes.
• Isopycnic centrifugation of viruses, plasmids, and high molecular weight DNA/RNA.
• Preparation of gradients for analytical ultracentrifugation studies.
• Component in diagnostic kits and reagents for clinical laboratory procedures.
• Research tool in biochemistry, cell biology, and molecular biology for fractionation studies.

Basic Information

Product Name	Metrizamide
CAS No.	31112-62-6
Molecular Formula	C18H22I3N3O8
Molecular Weight	789.10 g/mol
Synonyms	2-[3-Acetamido-5-(N-methylacetamido)-2,4,6-triiodobenzamido]-2-deoxy-D-glucose; Amipaque; Metrizamide; Metrizamidum; Metrizamid; NSC-107570; SH H 332 AB; 2-Deoxy-2-[[3-(N-methylacetamido)-5-acetamido-2,4,6-triiodobenzoyl]amino]-D-glucopyranose
EINECS	250-468-5

Quality Control

Our Metrizamide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards and can provide material traceable to primary reference standards upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.1%
pH (5% solution)	5.0 - 7.0
Biological Testing	Sterility and/or endotoxin levels available upon request

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.