Description

Diammonium 2,2'-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonate) CAS NO 30931-67-0 is a high-purity, water-soluble salt form of the widely used chromogenic substrate ABTS. This compound is essential for generating a stable, intensely colored radical cation, making it a critical reagent for enzymatic and biochemical assays. It is primarily utilized by research institutions, diagnostic kit manufacturers, and quality control laboratories in the life sciences and biotechnology sectors for applications such as antioxidant capacity testing and immunoassays.

Application

• Enzymatic Assays: As a substrate for peroxidases (e.g., horseradish peroxidase, HRP) in ELISA, western blotting, and other immunoassay detection systems.
• Antioxidant Capacity Measurement: Key component in the Trolox Equivalent Antioxidant Capacity (TEAC) assay and other spectrophotometric methods to quantify antioxidant activity in food, pharmaceutical, and biological samples.
• Biochemical Research: Used in kinetic studies of enzyme activity, particularly for hydrogen peroxide-producing or -consuming enzymes.
• Diagnostic Kit Manufacturing: Incorporated as a stable and sensitive colorimetric reagent in commercial clinical and research diagnostic test kits.
• Food & Beverage Analysis: Employed for quality control testing to measure the total antioxidant content in products like wines, juices, and edible oils.
• Pharmaceutical Screening: Utilized in high-throughput screening (HTS) platforms to identify potential antioxidant or enzyme-inhibiting drug candidates.

Basic Information

Product Name	Diammonium 2,2'-Azino-Bis(3-Ethylbenzothiazoline-6-Sulfonate)
CAS No.	30931-67-0
Molecular Formula	C18H24N6O6S4 · 2NH3
Molecular Weight	548.68 g/mol (Free acid)
Synonyms	ABTS Diammonium Salt; Diammonium 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate); 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt; ABTS·2NH3; Diammonium 2,2'-azino-di(3-ethylbenzothiazoline-6-sulfonate); ABTS Salt; Peroxidase Substrate ABTS.
EINECS	Contact for details

Quality Control

Our Diammonium ABTS is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture.

Specification

Item	Specification
Appearance	Green crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Absorbance Ratio (A340/Amax)	≤ 0.05
Solubility (in Water)	Clear, green solution

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.