Description

Blasticidin S Hydrochloride is a potent antibiotic compound belonging to the nucleoside antibiotic class. It serves as a critical selective agent in molecular biology and cell culture for the maintenance of plasmids carrying the blasticidin S deaminase (bsr) resistance gene. This product is essential for researchers and manufacturers in the biotechnology and pharmaceutical industries, particularly for applications in stable cell line development and gene expression studies.

Application

• Stable Cell Line Selection: Used as a selective agent in mammalian, plant, and bacterial cell culture systems to maintain transfected plasmids.
• Molecular Biology Research: Essential for experiments involving gene knockdown, overexpression, and functional genomics where blasticidin resistance is a selectable marker.
• Biopharmaceutical Production: Employed in the development and maintenance of producer cell lines for recombinant protein and monoclonal antibody manufacturing.
• Antifungal Agent: Exhibits activity against certain fungi and has been studied in agricultural contexts for plant disease control.
• Virology Studies: Utilized in the selection of viral vectors and the generation of stable packaging cell lines for viral production.
• High-Throughput Screening (HTS): Serves as a selection component in cell-based assays for drug discovery and toxicology testing.

Basic Information

Product Name	Blasticidin S Hydrochloride
CAS No.	3513-03-9
Molecular Formula	C17H26N8O5 • HCl
Molecular Weight	458.90 g/mol
Synonyms	Blasticidin S HCl; Blasticidin S Hydrochloride Salt; Bla-S; Antibiotic Bla-S; NSC 325319; 1-((4-Amino-2-oxo-1(2H)-pyrimidinyl)carbonyl)-3-(3-amino-5-methyl-2-oxo-1(2H)-pyridinyl)-L-alanine, Hydrochloride; Blasticidin S Monohydrochloride
EINECS	Contact for details

Quality Control

Our Blasticidin S Hydrochloride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and tests for residual solvents and endotoxins where applicable. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to maintain stability and potency. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
pH (1% Solution)	4.0 - 6.0
Endotoxin (LAL)	< 10 EU/mg (if applicable)
Biological Activity	Confirmed by cell culture assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.