Description

3-Nitrophenyl-β-D-Galactopyranoside is a chromogenic substrate specifically designed for the detection and assay of β-galactosidase enzyme activity. This compound matters for its critical role in biochemical research, molecular biology, and diagnostic applications, where precise enzymatic measurement is required. It is primarily needed by manufacturers of diagnostic kits, research institutions, and pharmaceutical companies involved in gene expression studies, cell-based assays, and microbiology.

Application

• Biochemical Research: As a specific substrate for the spectrophotometric or colorimetric assay of β-galactosidase activity in enzyme kinetics and inhibition studies.
• Molecular Biology: Used in reporter gene assays (e.g., lacZ gene) to monitor gene expression and promoter activity in bacterial, yeast, and mammalian cell systems.
• Diagnostic Manufacturing: A key component in the formulation of culture media and diagnostic kits for the identification of microorganisms, particularly in clinical and food safety testing.
• Cell & Tissue Staining: Employed in histochemistry and cytochemistry for the localization of β-galactosidase activity in cells and tissue sections.
• High-Throughput Screening (HTS): Utilized in pharmaceutical and biotechnology labs for drug discovery screens targeting β-galactosidase or related pathways.
• Enzyme Purification: Acts as an affinity ligand or activity tracer during the purification and characterization of β-galactosidase enzymes.

Basic Information

Product Name	3-Nitrophenyl-β-D-Galactopyranoside
CAS No.	3150-25-2
Molecular Formula	C12H15NO8
Molecular Weight	301.25 g/mol
Synonyms	3-Nitrophenyl β-D-Galactopyranoside; p-Nitrophenyl β-D-Galactopyranoside (common); 4-Nitrophenyl β-D-galactopyranoside; PNPG; ONPG (o-Nitrophenyl-β-D-galactopyranoside is a different isomer); β-D-Galactopyranoside, 3-nitrophenyl; Galactopyranoside, 3-nitrophenyl-β-D-; 3-NPG
EINECS	221-571-5

Quality Control

Our 3-Nitrophenyl-β-D-Galactopyranoside is produced under a strict quality management system. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.