Description

4-Hydroxyphenobarbital is a key pharmaceutical intermediate and metabolite of the barbiturate class. This compound matters for its critical role in analytical reference standards, metabolic studies, and the synthesis of specialized active pharmaceutical ingredients (APIs). It is primarily needed by pharmaceutical R&D laboratories, contract manufacturing organizations (CMOs), and manufacturers of diagnostic reagents and analytical standards.

Application

• Primary reference standard for the analytical quantification of phenobarbital and its metabolites in pharmaceutical quality control (QC) and bioanalytical studies.
• Critical intermediate in the research-scale synthesis of novel barbiturate derivatives and specialized pharmaceutical compounds.
• Metabolite standard for clinical and forensic toxicology testing, enabling accurate drug monitoring and compliance testing.
• Key reagent in the development and validation of diagnostic assays and kits for therapeutic drug monitoring (TDM).
• Building block for pharmacological research investigating the metabolism, excretion pathways, and structure-activity relationships (SAR) of barbiturates.
• Used in academic and industrial research focused on cytochrome P450 enzyme activity and drug-drug interaction studies.

Basic Information

Product Name	4-Hydroxyphenobarbital
CAS No.	379-34-0
Molecular Formula	C12H12N2O4
Molecular Weight	248.24 g/mol
Synonyms	5-Ethyl-5-(4-hydroxyphenyl)-1,3-diazinane-2,4,6-trione; 5-Ethyl-5-(p-hydroxyphenyl)barbituric Acid; Phenobarbital 4-Hydroxy Metabolite; 4'-Hydroxyphenobarbital; p-Hydroxyphenobarbital; NSC 526063; 5-(4-Hydroxyphenyl)-5-ethylbarbituric Acid
EINECS	Contact for details

Quality Control

Our 4-Hydroxyphenobarbital is produced and tested under a rigorous quality management system. Each batch undergoes comprehensive analysis to ensure high purity and consistency, meeting the exacting standards required for pharmaceutical research and reference materials. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting identity, purity, and impurity profiles.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.