Description

N,N,N',N'-Tetramethylbenzidine (TMB) is a high-purity chromogenic substrate widely recognized for its critical role in enzymatic detection systems. Its primary value lies in producing a distinct, measurable blue color upon oxidation, enabling sensitive and quantitative analysis in diagnostic and research applications. This compound is essential for industries and laboratories requiring precise colorimetric endpoints, particularly in clinical diagnostics, life science research, and immunoassay development.

Application

• Clinical Diagnostics: As a key chromogen for horseradish peroxidase (HRP) in ELISA (Enzyme-Linked Immunosorbent Assay) kits for detecting antigens, antibodies, and biomarkers.
• Life Science Research: Used in Western blotting, immunohistochemistry (IHC), and other blotting techniques for visualizing protein bands or cellular targets.
• Point-of-Care Testing (POCT): Employed in lateral flow assays and rapid test strips for applications such as pregnancy tests, infectious disease detection, and cardiac marker tests.
• Environmental & Food Safety Testing: Applied in enzymatic assays for detecting contaminants like pesticides, pathogens, or toxins.
• Biochemical Assay Development: Serves as a standard substrate in developing and validating new enzymatic assays and biosensors.
• Forensic Analysis: Utilized in certain presumptive tests for the detection of biological fluids.

Basic Information

Product Name	N,N,N',N'-Tetramethylbenzidine
CAS No.	366-29-0
Molecular Formula	C16H20N2
Molecular Weight	240.34 g/mol
Synonyms	TMB; 3,3',5,5'-Tetramethylbenzidine; Benzidine, 3,3',5,5'-tetramethyl-; 4,4'-Diamino-3,3',5,5'-tetramethylbiphenyl; 3,3',5,5'-Tetramethyl-[1,1'-biphenyl]-4,4'-diamine; TMB Substrate; TMB Free Base; TMB Chromogen
EINECS	206-679-7

Quality Control

Our N,N,N',N'-Tetramethylbenzidine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation, to meet stringent in-house specifications. A Certificate of Analysis (COA) detailing all test results is provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, well-ventilated area at room temperature (15-25°C). This material is light-sensitive (store away from light) and easily oxidized (store under inert atmosphere). Keep away from strong oxidizing agents.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 99.0%
Melting Point	168 - 172 °C
Loss on Drying	≤ 0.5 %
Residue on Ignition	≤ 0.1 %
Solubility	Soluble in DMSO, ethanol, and acetic acid

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.