Description

Phenylmethylsulfonyl Fluoride (PMSF) is a potent and irreversible serine protease inhibitor widely used in biochemical and pharmaceutical research. Its primary value lies in its ability to effectively inhibit enzymes like trypsin, chymotrypsin, and thrombin, thereby preventing unwanted protein degradation during cell lysis and protein purification. This compound is an essential reagent for researchers in proteomics, molecular biology, and drug discovery, as well as for quality control in biopharmaceutical production processes.

Application

• Protease Inhibition in Protein Research: Critical for preventing proteolytic degradation during the extraction and purification of proteins from tissues and cell cultures.
• Cell Lysis Buffer Component: A standard additive to RIPA and other lysis buffers to maintain protein integrity for Western blotting, ELISA, and immunoprecipitation.
• Biopharmaceutical Manufacturing: Used in downstream processing to control protease activity and ensure the stability of therapeutic proteins and antibodies.
• Enzyme Activity Studies: Employed as a specific inhibitor to study the function and mechanism of serine proteases in biochemical assays.
• Diagnostic Reagent Formulation: Incorporated into diagnostic kits and reagents to stabilize enzyme components and ensure consistent performance.
• Academic and Industrial R&D: A fundamental tool in life science research for investigating apoptosis, signal transduction pathways, and protein function.

Basic Information

Item	Details
Product Name	Phenylmethylsulfonyl Fluoride
CAS No.	329-98-6
Molecular Formula	C7H7FO2S
Molecular Weight	174.19 g/mol
Synonyms	PMSF; α-Toluenesulfonyl fluoride; Benzenemethanesulfonyl fluoride; 4-Methylbenzenesulfonyl fluoride (isomer reference); Tosyl fluoride (related); Protease Inhibitor PMSF; Serine protease inhibitor; Benzylsulfonyl fluoride
EINECS	206-350-2

Quality Control

Our Phenylmethylsulfonyl Fluoride is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, identity confirmation by IR and NMR, and residual solvent analysis, to ensure it meets high-purity standards suitable for research and industrial applications. A detailed Certificate of Analysis (COA) is provided with every shipment to guarantee traceability and consistent batch-to-batch quality.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. Keep away from strong bases.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.5%
Melting Point	90 - 94 °C
Loss on Drying	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.