Description

Tpck CAS NO 329-30-6 is a highly specific, irreversible inhibitor of serine proteases, most notably chymotrypsin. This compound is critical for biochemical research and pharmaceutical development, enabling precise control over enzymatic activity. It is primarily utilized by researchers in proteomics, structural biology, and by manufacturers developing enzyme-based therapeutics and diagnostic kits.

Application

• Protease Inhibition Studies: Used as a specific active-site titrant and inhibitor for chymotrypsin and related serine proteases in kinetic and mechanistic research.
• Protein Purification & Characterization: Employed to inactivate specific proteases during the isolation and analysis of proteins and peptides to prevent degradation.
• Pharmaceutical Research: Serves as a key tool compound for studying protease involvement in disease pathways and for screening potential therapeutic inhibitors.
• Diagnostic Kit Manufacturing: Incorporated into diagnostic assays and kits to control background protease activity, ensuring assay specificity and stability.
• Cell Biology & Biochemistry: Applied in cell culture and lysate experiments to probe the functional role of specific proteolytic enzymes.

Basic Information

Product Name	Tpck
CAS No.	329-30-6
Molecular Formula	C14H14ClNO2S
Molecular Weight	295.78 g/mol
Synonyms	N-Tosyl-L-phenylalanine chloromethyl ketone; TPCK; L-1-Chloro-3-tosylamido-4-phenyl-2-butanone; N-p-Tosyl-L-phenylalanyl chloromethane; Chymostatin; TLCK (related compound); Tosylphenylalanyl chloromethyl ketone; Protease Inhibitor TPCK
EINECS	206-340-5

Quality Control

Our Tpck is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, identity confirmation by IR spectroscopy, and specific rotation measurement. Certificates of Analysis (COA) detailing all specifications are provided to ensure reproducibility and reliability for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Specific Rotation	-30° to -34° (c=1 in methanol)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.