Description

Naphthol As-Bi β-D-Glucuronide is a specialized chromogenic substrate used in enzymatic detection systems. This compound is critical for histochemical and cytochemical staining, where it serves as a precursor for the formation of an insoluble, colored precipitate upon enzymatic cleavage. It is primarily utilized by research institutions, diagnostic laboratories, and pharmaceutical developers engaged in enzyme-linked assays and tissue analysis. The product offers high sensitivity and specificity for the detection of β-glucuronidase activity.

Application

• Histochemical Staining: Primary substrate for the localization of β-glucuronidase activity in tissue sections.
• Diagnostic Assays: Key component in diagnostic kits for detecting specific enzymatic markers in clinical samples.
• Biomedical Research: Used in laboratory studies to investigate enzyme function, gene expression, and cellular processes.
• Microbiology: Employed in microbial identification systems that rely on β-glucuronidase as a biochemical marker.
• Pharmaceutical Development: Utilized in drug discovery and toxicology studies to assess compound effects on enzymatic pathways.
• Plant Biology: Applied in studies of plant physiology and pathology to monitor β-glucuronidase reporter gene (GUS) activity.

Basic Information

Product Name	Naphthol As-Bi β-D-Glucuronide
CAS No.	37-87-6
Molecular Formula	C23H24NO9
Molecular Weight	458.44 g/mol
Synonyms	Naphthol AS-BI β-D-Glucopyranosiduronic Acid; 6-[(3-Hydroxy-2-naphthoyl)amino]-1-naphthyl β-D-Glucopyranosiduronic Acid; Naphthol AS-BI Glucuronide; AS-BI Glucuronide; Naphthol ASBI-β-D-Glucuronide; β-Glucuronidase Substrate; 3-Hydroxy-2-naphthanilide β-D-Glucuronide
EINECS	Contact for details

Quality Control

Our Naphthol As-Bi β-D-Glucuronide is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high purity suitable for sensitive diagnostic and research applications. Each lot is tested for identity, purity, and performance. A comprehensive Certificate of Analysis (COA) detailing specifications and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure containers are sealed tightly after each use to minimize exposure to atmospheric moisture and light.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	≤ 20 ppm
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.