Description

Pregn-4-Ene-3,20-Dione-2,2,4,6,6,21,21,21-D8, 11,17-Dihydroxy-, (11β)- is a high-purity deuterated analog of hydrocortisone, specifically designed for use as an internal standard in advanced analytical chemistry. This compound is critical for ensuring the accuracy and reliability of quantitative analyses, particularly in regulated environments where precision is non-negotiable. It is primarily utilized by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in method development, pharmacokinetic studies, and quality control for steroid-based therapeutics.

Application

• Internal Standard for LC-MS/MS: Essential for the precise quantification of endogenous and synthetic corticosteroids in complex biological matrices like plasma, serum, and urine.
• Pharmaceutical R&D: Used in method development and validation for the analysis of hydrocortisone and related steroid drugs, ensuring compliance with FDA and ICH guidelines.
• Metabolomics and Biomarker Discovery: Serves as a stable isotope-labeled tracer in metabolic pathway studies and the identification of steroid-based biomarkers.
• Clinical Research: Enables accurate measurement of cortisol levels in clinical studies related to stress, adrenal disorders, and endocrine function.
• Forensic Toxicology: Employed in confirmatory testing for the presence of exogenous corticosteroids in anti-doping controls and forensic investigations.
• Reference Material: Acts as a certified reference material (CRM) for calibrating analytical instruments and ensuring traceability in measurements.

Basic Information

Product Name	Pregn-4-Ene-3,20-Dione-2,2,4,6,6,21,21,21-D8, 11,17-Dihydroxy-, (11β)-
CAS No.	2479914-04-8
Molecular Formula	C₂₁H₂₂D₈O₅
Molecular Weight	370.50 g/mol (approx.)
Synonyms	Deuterated Hydrocortisone (d8); [2,2,4,6,6,21,21,21-²H₈]-Hydrocortisone; 11β,17,21-Trihydroxypregn-4-ene-3,20-dione-d8; Cortisol-d8; (11β)-11,17,21-Trihydroxypregn-4-ene-3,20-dione-2,2,4,6,6,21,21,21-d8; 4-Pregnene-11β,17α,21-triol-3,20-dione-d8; Stable Isotope Labeled Hydrocortisone
EINECS	Contact for details

Quality Control

Every batch of our deuterated hydrocortisone is produced and analyzed under strict quality management systems. We guarantee high chemical and isotopic purity, verified by advanced spectroscopic and chromatographic techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing batch-specific data on purity, isotopic enrichment, and solvent residues. Our production adheres to standards suitable for pharmaceutical reference material applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C (refrigerated) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is sealed in a dry environment after each use to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Enrichment	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.