Description

Pyridostigmine-D6 Bromide is a deuterium-labeled analog of the acetylcholinesterase inhibitor pyridostigmine bromide, serving as a critical internal standard for precise quantitative analysis. This high-purity stable isotope-labeled compound is essential for ensuring accuracy and reliability in advanced analytical techniques, particularly in regulated environments. It is primarily required by pharmaceutical R&D laboratories, contract research organizations (CROs), and analytical testing facilities engaged in method development, pharmacokinetic studies, and bioanalytical research for the parent drug.

Application

• Internal Standard for LC-MS/MS: Used as a quantitative reference standard in Liquid Chromatography-Tandem Mass Spectrometry for the analysis of pyridostigmine in biological matrices like plasma and serum.
• Pharmacokinetic and Metabolism Studies: Enables accurate tracking and measurement of drug absorption, distribution, metabolism, and excretion (ADME) in preclinical and clinical research.
• Bioanalytical Method Development and Validation: Critical for developing, optimizing, and validating robust analytical methods in compliance with FDA, EMA, and ICH guidelines.
• Drug Impurity Profiling and Stability Testing: Assists in identifying and quantifying potential degradants or related substances in drug substance and product testing.
• Forensic and Doping Control Analysis: Supports accurate quantification in forensic toxicology and anti-doping control programs where precise measurement is legally mandated.

Basic Information

Product Name	Pyridostigmine-D6 Bromide
CAS No.	2375858-08-3
Molecular Formula	C₉H₁₇D₆BrN₂O₂
Molecular Weight	289.21 g/mol (approx.)
Synonyms	3-[(Dimethylamino)carbonyl]oxy-1-methylpyridin-1-ium-2,4,5,6-d4 bromide (N-methyl-d3); Pyridostigmine-d6 Bromide; 1-Methyl-3-hydroxy-pyridinium-d6 Bromide Dimethylcarbamate; Deuterated Pyridostigmine Bromide; Mestinon-d6 Bromide; (3-Hydroxy-1-methylpyridin-1-ium-2,4,5,6-d4) dimethylcarbamate bromide; 3-[[(Dimethyl-d6)amino]carbonyl]oxy-1-methylpyridinium bromide.
EINECS	Contact for details

Quality Control

Every batch of Pyridostigmine-D6 Bromide is manufactured and analyzed under strict quality management systems to ensure the highest isotopic and chemical purity required for analytical applications. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) detailing batch-specific results for isotopic purity, chemical purity (by HPLC), and identity confirmation (by NMR and MS) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term storage, consider desiccant and inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (Deuterium Incorporation)	≥98.0 atom % D
Chemical Purity (by HPLC)	≥95.0%
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Complies with ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.