Description

Diketone-Peg4-Biotin is a high-purity, heterobifunctional crosslinking reagent featuring a diketone group and a biotin moiety connected by a tetraethylene glycol (PEG4) spacer. This molecular design is critical for creating stable, site-specific conjugations in advanced bioconjugation and labeling workflows. It is an essential tool for researchers and developers in pharmaceutical R&D, proteomics, and diagnostic assay development who require precise and efficient protein or antibody modification.

Application

• Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of cytotoxic payloads to antibodies via engineered ketone handles.
• Protein and Peptide Labeling: Biotinylation of biomolecules for detection, purification, or immobilization using streptavidin-based systems.
• Diagnostic Reagent Manufacturing: Production of biotinylated detection antibodies or antigens for ELISA, lateral flow assays, and other immunoassays.
• Affinity Probe Construction: Synthesis of activity-based probes (ABPs) or photoaffinity labels for target identification and validation in chemical biology.
• Surface Functionalization: Modification of biosensor chips, nanoparticles, or other solid supports for creating oriented capture surfaces.
• Pull-Down Assays: Immobilization of bait proteins or small molecules for studying protein-protein or protein-ligand interactions.

Basic Information

Product Name	Diketone-Peg4-Biotin
CAS No.	2353409-85-3
Molecular Formula	C31H51N5O11S
Molecular Weight	701.83 g/mol
Synonyms	1-(2-(2-(2-(2-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethoxy)ethoxy)-3-oxobutane-1,2-dione; Biotin-PEG4-diketone; Diketone-PEG4-Biotin Reagent; Biotinylated Diketone Crosslinker; PEG4-Spaced Biotin-Diketone; Ketone-Biotin Heterobifunctional Linker; Biotin-PEG4-acetylacetone
EINECS	Contact for details

Quality Control

Every batch of Diketone-Peg4-Biotin is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request, providing detailed results for identity, purity, and functional suitability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Activity	Conforms (by conjugation test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.