Description

Gly-Gly-Gly-Peg3-Tco is a specialized tripeptide-PEG-conjugate featuring a trans-cyclooctene (TCO) functional group. This compound is engineered for high-efficiency bioorthogonal click chemistry, specifically inverse electron demand Diels-Alder (IEDDA) reactions with tetrazine derivatives. It is essential for researchers and manufacturers in the fields of bioconjugation, targeted drug delivery, and advanced proteomics who require precise, rapid, and biocompatible labeling strategies. The molecule combines the biocompatibility of a glycine-based peptide linker with the flexibility of a polyethylene glycol (PEG) spacer and the rapid kinetics of the TCO moiety.

Application

• Bioconjugation & Antibody-Drug Conjugate (ADC) Development: Site-specific conjugation of payloads (e.g., toxins, fluorophores, drugs) to antibodies, proteins, or peptides via IEDDA click chemistry.
• Pre-Targeting Strategies in Radioimmunotherapy & Imaging: Enables multi-step pre-targeting approaches for improved tumor-to-background ratios in diagnostic and therapeutic applications.
• Proteomics & Biomarker Labeling: Selective and rapid labeling of biomolecules in complex biological mixtures for mass spectrometry analysis or fluorescence detection.
• Surface Functionalization & Material Science: Modification of nanoparticles, hydrogels, or diagnostic devices with bioactive ligands for targeted interactions.
• Live-Cell Imaging & Tracking: Real-time labeling of cellular components with minimal perturbation due to the fast kinetics and bioorthogonality of the TCO-tetrazine reaction.
• Chemical Biology Tool Development: A key building block for creating novel probes and tools to study biological processes in vivo and in vitro.

Basic Information

Product Name	Gly-Gly-Gly-Peg3-Tco
CAS No.	2353409-81-9
Molecular Formula	C27H46N4O10
Molecular Weight	586.68 g/mol
Synonyms	Glycyl-glycyl-glycyl-3,6,9,12-tetraoxapentadecan-15-oic acid-(E)-cyclooct-4-en-1-yl ester; GGG-PEG3-TCO; Triglycine-PEG3-trans-cyclooctene; TCO-PEG3-GGG; (E)-Cyclooct-4-en-1-yl 3,6,9,12-tetraoxa-2-azapentadecan-15-oate glycine-glycine-glycine conjugate; trans-Cyclooctene-PEG3-triglycine; IEDDA Click Chemistry Reagent
EINECS	Contact for details

Quality Control

Our Gly-Gly-Gly-Peg3-Tco is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment. We support compliance with cGMP and ISO 9001 standards for critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; partially soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.