Description

n-(Dbco-Peg4)-n-Biotin-Peg4-Nhs CAS NO 2353409-72-8 is a heterobifunctional crosslinker designed for advanced bioconjugation and labeling applications. This compound integrates a DBCO (dibenzocyclooctyne) group for strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry, a biotin moiety for high-affinity streptavidin binding, and an NHS ester for efficient amine coupling. It is essential for researchers and developers in life sciences and diagnostics who require precise, multi-step labeling strategies without the need for metal catalysts. This reagent enables the creation of stable, site-specific bioconjugates for targeted drug delivery, in vitro diagnostics, and advanced biomaterial engineering.

Application

• Bioconjugation and Protein Labeling: Site-specific conjugation of antibodies, enzymes, or other proteins with biotin tags for detection and purification via streptavidin systems.
• Diagnostic Assay Development: Construction of sensitive immunoassay and biosensor platforms by linking detection molecules to solid supports or signal amplifiers.
• ADC (Antibody-Drug Conjugate) Research: Facilitating the development of novel ADC linkers by enabling controlled, multi-functional attachment of payloads to targeting antibodies.
• Cell Surface Engineering and Imaging: Labeling of cell surface proteins or glycans for flow cytometry, super-resolution microscopy, and in vivo imaging studies.
• Nucleic Acid Probe Labeling: Modification of oligonucleotides (e.g., primers, probes) for fluorescence in situ hybridization (FISH) or microarray applications.
• Biomaterial and Hydrogel Functionalization: Incorporation of bioactive ligands into polymer scaffolds for tissue engineering and controlled release systems.
• Affinity Chromatography Resin Preparation: Immobilization of biotinylated ligands onto streptavidin-coated beads for high-purity protein isolation.
• Small Molecule Drug Conjugation: Creating biotinylated drug derivatives for pharmacokinetic studies, target engagement assays, and prodrug strategies.

Basic Information

Product Name	n-(Dbco-Peg4)-n-Biotin-Peg4-Nhs
CAS No.	2353409-72-8
Molecular Formula	C58H86N6O18S
Molecular Weight	1187.4 g/mol
Synonyms	DBCO-PEG4-Biotin-PEG4-NHS ester; DBCO-PEG4-Biotin-PEG4-NHS; N-(DBCO-PEG4)-N-Biotin-PEG4-NHS; DBCO-PEG4-Biotin-PEG4-N-hydroxysuccinimide ester; Biotin-PEG4-DBCO-PEG4-NHS; 1-(DBCO-PEG4)-1-(Biotin-PEG4)-NHS; Dibenzocyclooctyne-PEG4-Biotin-PEG4-NHS; DBCO-PEG4-Biotin-PEG4-SE
EINECS	Contact for details

Quality Control

Our n-(Dbco-Peg4)-n-Biotin-Peg4-Nhs is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical characterization to ensure identity, purity, and functional integrity. We provide a detailed Certificate of Analysis (COA) with every shipment, which includes data from HPLC, NMR, and mass spectrometry analysis. Our quality commitment ensures the reagent performs reliably in your most sensitive click chemistry and bioconjugation applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with molecular structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (NHS Ester)	≥ 90% active ester by amine consumption assay
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.