Description

Flurescein-Peg5-Nhs Ester is a high-purity, amine-reactive fluorescent labeling reagent designed for bioconjugation. This compound is critical for creating stable, fluorescently tagged biomolecules for advanced detection and imaging applications. It is primarily used by researchers and manufacturers in the fields of biopharmaceutical development, diagnostic assay production, and life science research, where precise and reliable labeling is essential.

Application

• Antibody-Drug Conjugate (ADC) Development: For site-specific conjugation of fluorescent dyes to antibodies for tracking and characterization.
• Diagnostic Reagent Manufacturing: As a key component in the synthesis of fluorescent probes for immunoassays and lateral flow tests.
• Protein & Peptide Labeling: Enables the introduction of a fluorescein fluorophore onto proteins, peptides, and other amine-containing biomolecules for fluorescence microscopy (FLM) and flow cytometry.
• Biomaterial Functionalization: Used to modify surfaces of nanoparticles, polymers, or hydrogels with fluorescent markers for in vitro and in vivo tracking studies.
• Oligonucleotide Probes: Facilitates the synthesis of fluorescently labeled DNA or RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cellular Imaging Reagents: A building block for creating cell-permeable fluorescent tags to visualize intracellular targets.

Basic Information

Product Name	Flurescein-Peg5-Nhs Ester
CAS No.	2353409-62-6
Molecular Formula	C46H55N3O17
Molecular Weight	922.0 g/mol (approx.)
Synonyms	5(6)-FAM-PEG5-NHS Ester; Fluorescein-5(6)-PEG5-NHS Ester; 5(6)-Carboxyfluorescein-PEG5-NHS Ester; 6-FAM SE-PEG5; Fluorescein-Pentaethylene glycol-N-hydroxysuccinimide ester; 5-FAM-PEG5-NHS; 6-FAM-PEG5-NHS; 5(6)-FAM, SE-PEG5
EINECS	Contact for details

Quality Control

Our Flurescein-Peg5-Nhs Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure it meets the high standards required for sensitive bioconjugation work. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95%
Single Isomer Content (5- or 6- isomer)	≥ 90%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.