Description

Dde Biotin-Peg4-Tamra-Peg4 Alkyne is a multifunctional, heterobifunctional crosslinker designed for site-specific bioconjugation via sequential click chemistry and deprotection strategies. It integrates a Dde (1-(4,4-dimethyl-2,6-dioxocyclohexylidene)ethyl) protecting group, a biotin affinity tag, two hydrophilic PEG4 spacers, a TAMRA (carboxytetramethylrhodamine) fluorescent reporter, and a terminal alkyne for copper-catalyzed or strain-promoted azide–alkyne cycloaddition (CuAAC/SPAAC). This molecular architecture enables precise, modular labeling of biomolecules—particularly in live-cell imaging, target validation, and multi-step proteomics workflows—where orthogonal reactivity, reduced steric hindrance, and dual detection modalities are critical. It is essential for research-intensive laboratories in academic institutions, biopharmaceutical R&D, and diagnostic assay development.

Application

• Live-cell fluorescent labeling of azide-modified proteins or glycans using SPAAC or CuAAC, followed by Dde deprotection and streptavidin-based signal amplification
• Multi-step target engagement studies combining biotin-streptavidin pull-down with TAMRA fluorescence readout for spatial-temporal tracking
• Development of homogeneous TR-FRET (time-resolved Förster resonance energy transfer) assays leveraging TAMRA as donor/acceptor and biotin for immobilization
• Construction of cleavable, traceable antibody–drug conjugate (ADC) intermediates with controlled release via Dde deprotection under mild acidic conditions
• Proteome-wide chemoproteomic profiling using competitive activity-based protein profiling (ABPP) with dual-readout validation
• Surface functionalization of PEGylated nanoparticles for targeted delivery and real-time biodistribution imaging
• Preparation of standardized reference standards for LC-MS/MS method validation in bioanalytical labs

Basic Information

Product Name	Dde Biotin-Peg4-Tamra-Peg4 Alkyne
CAS No.	2353409-55-7
Molecular Formula	C68H87N11O21
Molecular Weight	1342.47 g/mol
Synonyms	Dde-Biotin-PEG4-TAMRA-PEG4-Alkyne; Dde-Biotin-PEG4-TMR-PEG4-Alkyne; Biotin-PEG4-TAMRA-PEG4-Alkyne (Dde-protected); 1-(4,4-Dimethyl-2,6-dioxocyclohexylidene)ethyl-biotin-PEG4-TAMRA-PEG4-propargyl; Dde-Biotin-(CH2CH2O)4-TAMRA-(CH2CH2O)4-alkyne; Dde-Biotin-PEG24-TAMRA-PEG24-Alkyne (alternative nomenclature); Dde-Biotin-PEG4-Rhodamine B-PEG4-Alkyne (TAMRA analog)
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. Each batch is verified for identity (HPLC retention time match and MS confirmation), purity (≥95% by HPLC), and absence of critical impurities (e.g., residual solvents, heavy metals, and unreacted starting materials). Testing adheres to ACS Reagent Grade and ISO 9001:2015 quality management system requirements. Custom QC protocols—including endotoxin testing (<0.1 EU/mg) and sterility verification—are available for GMP-aligned applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2–8 °C under inert atmosphere (argon or nitrogen) to prevent oxidation and moisture uptake. Due to its hygroscopic nature, minimize exposure to ambient humidity; allow vial to equilibrate to room temperature in a desiccator before opening. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Red to dark red lyophilized solid
Identity (HPLC)	Retention time matches reference standard (±0.2 min)
Identity (MS)	[M+H]+ m/z = 1343.4 ± 0.3 (calculated for C68H88N11O21)
Assay (HPLC)	≥95.0%
Related Substances (HPLC)	Individual impurity ≤1.0%; Total impurities ≤3.0%
Residual Solvents (GC)	MeOH ≤0.3%, DCM ≤0.06%, DMF ≤0.5%
Water Content (KF)	≤2.0%
pH (5 mg/mL in water)	5.5–7.0

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.