Description

Biotin-Peg36-Pfp Ester CAS NO 2353409-51-3 is a high-purity, heterobifunctional polyethylene glycol (PEG) crosslinker designed for advanced bioconjugation and chemical biology applications. Its core value lies in enabling the precise, site-specific attachment of biotinylated molecules to target biomolecules or surfaces via its reactive pentafluorophenyl (PFP) ester group, while the extended, hydrophilic PEG36 spacer significantly enhances solubility and reduces steric hindrance. This compound is essential for researchers and manufacturers in pharmaceutical development, proteomics, and diagnostic assay creation who require reliable and efficient conjugation strategies.

Application

• ADC (Antibody-Drug Conjugate) Development: Site-specific conjugation of cytotoxic payloads or imaging agents to antibodies via lysine residues.
• Biosensor and Diagnostic Chip Fabrication: Immobilization of biotinylated proteins, antibodies, or nucleic acids onto streptavidin-coated surfaces for assay development.
• Protein and Peptide Labeling: Introduction of a biotin tag onto proteins or peptides for purification (streptavidin affinity) or detection (ELISA, Western Blot).
• Polymer-Protein Conjugate Synthesis: Creating PEGylated biotherapeutics to improve pharmacokinetic profiles, such as increased circulation half-life and reduced immunogenicity.
• Chemical Biology Probes: Construction of activity-based probes (ABPs) or pull-down reagents for target identification and validation in drug discovery.
• Nanoparticle Functionalization: Surface modification of liposomes, dendrimers, or other nanocarriers with targeting ligands for drug delivery systems.
• Materials Science: Crafting biofunctional hydrogels or surfaces with specific biological activity for tissue engineering and cell culture applications.

Basic Information

Product Name	Biotin-Peg36-Pfp Ester
CAS No.	2353409-51-3
Molecular Formula	CnHmF5N2OpS (Contact for details)
Molecular Weight	~2500 g/mol (Contact for exact value)
Synonyms	Biotin-PEG36-PFP Ester; Biotin-PEG36-Pentafluorophenyl Ester; Biotin-PEG36-OSu (alternative naming); Biotinylated PEG PFP Ester (MW ~2500); 5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2,5,8,11,14,17,20,23,26,29,32,35,38,41,44,47,50,53,56,59,62,65,68,71,74,77,80,83,86,89,92,95,98,101,104,107-decaoxa-110,111,112,113,114-pentafluoro-116-oxohexatriaconta-1,115-dien-116-yl)pentanamide; PFP-PEG36-Biotin; Heterobifunctional PEG Crosslinker, Biotin-PFP.
EINECS	Contact for details

Quality Control

Our Biotin-Peg36-Pfp Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (NMR, MS), to ensure it meets the high standards required for sensitive bioconjugation work. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing batch-specific results for critical parameters such as assay purity, functional group reactivity, and moisture content. Our commitment to cGMP-grade practices ensures consistency and reliability for your research and development pipelines.

Storage

Preserve in a tightly closed container, protected from light. Due to its hygroscopic (moisture-sensitive) nature, store the product in a dry environment, ideally under an inert atmosphere (argon or nitrogen) for long-term stability. For optimal shelf life, store at -20°C ± 5°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active ester.

Specification

Item	Specification
Appearance	White to off-white solid or viscous liquid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (PFP Ester)	≥ 90% active
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.